Publications by authors named "N T Wolfman"
Article Synopsis
- Chronic mucocutaneous candidiasis disease (CMCD) involves frequent infections of the skin, nails, and mucous membranes primarily caused by Candida albicans in otherwise healthy individuals.
- Two genetic causes of CMCD were identified: one being a complete deficiency in the IL-17 receptor A (IL-17RA), and the other a partial deficiency in interleukin-17F (IL-17F).
- This research highlights that IL-17A and IL-17F play a crucial role in protecting against Candida infections, while their absence reveals that they aren’t necessary for overall immune function.
Distinct in vitro binding properties of the anti-CD20 small modular immunopharmaceutical 2LM20-4 result in profound and sustained in vivo potency in cynomolgus monkeys.
Rheumatology (Oxford)
June 2011
Objectives: To characterize the in vitro binding and effector function properties of CD20-directed small modular immunopharmaceutical (SMIP) 2LM20-4, and to compare its in vivo B-cell depletion activity with the mutated 2LM20-4 P331S [no in vitro complement-dependent cytotoxicity (CDC)] and rituximab in cynomolgus monkeys.
Methods: Direct binding is examined in flow cytometry, confocal microscopy, scatchard and lipid raft assays. Effector function assays include CDC and Fc-mediated cellular toxicity.
Vanin-1 is a pantetheinase that catalyzes the hydrolysis of pantetheine to produce pantothenic acid (vitamin B5) and cysteamine. Reported here is a highly sensitive fluorescent assay using a novel fluorescently labeled pantothenate derivative. The assay has been used for characterization of a soluble version of human vanin-1 recombinant protein, identification and characterization of hits from high-throughput screening (HTS), and quantification of vanin pantothenase activity in cell lines and tissues.
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