[Content of some pro- and anti-inflammatory cytokines in blood sera of leptospirosis patients].

Aim: Study the content of some pro- and anti-inflammatory cytokines in blood sera of leptospirosis patients in dynamics of infectious process and the role of these cytokines in the disease immunopathogenesis.

Materials And Methods: The content of cytokines in blood sera was determined by a method based on xMAP technology with a standard panel consisting of 9 analytes: TNF-α, MCP-1, IL-8, IL-4, IL-6, IL-10, IL-1Ra, IL-12 (p70), IFN-γ.

Results: A significantly increased level of IL-8, IL-10, TNF-α was confirmed and the increased content of MCP-1 in leptospirosis patients compared with practically healthy donors was established for the first time.

[Development of PCR test system based on colA gene for detection of leptospirae in clinical material].

Aim: Development of primers for the detection of leptospirae in clinical material including urine.

Materials And Methods: Study of specificity and sensitivity of primers complementary to colA gene in standard PCR by using DNA preparation of cultures of pathogenic and saprophytic leptospirae, biological materials from healthy humans and dogs, including contaminated with pathogenic leptospirae culture.

Results: Specific interaction of these primers with DNA of pathogenic leptospirae of 14 serogroups was established.

[Secondary leptospirosis].

The author describes cases of secondary leptospirosis. Secondary infection with leptospiras occurred in patients with concomitant pathology. In connection with occupational activity or life-style the patients got infected from Rattus norvegicus.

[Radionuclide ventriculography in the diagnosis of a postinfarct aneurysm and the evaluation of its impact on left ventricular function].

This is a report on thirty-four patients subjected to radionuclide ventriculography because of suspected post-infarction aneurysm. To discover eventual kinetic disorders, apart from visual assessment of the wall kinetics, amplitude-phase analysis is also employed. The sensitivity of the method is compared with that of bi-dimensional echocardiography.