Use of cyclodextrins for the enantioselective separation of ergot alkaloids by capillary zone electrophoresis.

Ergot alkaloid enantiomer derivatives were resolved using capillary zone electrophoresis. The effect of cyclodextrins, added to the background electrolyte, on the migration time and the resolution was studied. Good separation for epimeric ergot alkaloid derivatives was also obtained using phosphate buffer at pH 2.

Mutant strains of Streptomyces cinnamonensis protoplasts. Cultural and physiological conditions.

Mycelium of Streptomyces cinnamonensis mutant strains cultivated in a synthetic medium with glycine produced protoplasts after lysis of cell walls with lysozyme. The protoplast yield was up to 95%. The protoplasts could revert and mycelial forms were thus regenerated.

Relationship between the fatty acid composition and the type of antibiotics produced by Streptomyces lasaliensis.

The GC-MS method was used to analyze fatty acids in a parent strain of Streptomyces lasaliensis and its two mutants producing only lasalocid or quinomycin. The biosynthesis of these substances bears relation to differences in the relative proportion of fatty acids. Biosynthetic relationships were revealed between the starter fatty acid units, their precursors, i.

Production of quinomycin A in Streptomyces lasaliensis.

In addition to lasalocid, an oligoether coccidiostatic compound, other compounds are synthesized by Streptomyces lasaliensis. Mutants producing either of two antibiotics, lasalocid A or quinomycin A (an antibiotic of quinoxaline character), were obtained by natural selection and by mutagenesis. Methods of isolation, purification and estimation of both compounds were established.

Microbial glucosidation of 1,2,4-trihydroxy-9,10-anthraquinone (purpurin).

Streptomyces aureofaciens glucosidizes 1,2,4-trihydroxy-9,10-anthraquinone (purpurin) added to the cultivation medium to yield the corresponding 2-beta-D-glucoside. The identity of the glucoside was demonstrated by comparing its physico-chemical properties with data of an authentic sample prepared synthetically. A further chemical glucosidation of the acetylated 2-beta-D-glucoside gives rise to 2-(hepta-O-acetyl-beta-gentiobiosyl)-4-(tetra-O-acetyl-beta-D-gluc opyranosyl) purpurin.