Publications by authors named "N Schuurbiers"
Glucose metabolism of the bovine embryo is low during the first cleavages and increases sharply after the major resumption of the genome (8-16 cells). The mRNA level for genes involved in glucose metabolism was tested by RT-PCR on individual oocytes and embryos at different stages of development. These genes were: glucose transport GLUT-1, hexokinase (HK), glucose-6-phosphatase-dehydrogenase (G6PDH), and glucose-phosphate-isomerase (GPI); actin was used as a reference transcript.
View Article and Find Full Text PDFArticle Synopsis
- Researchers studied how fetal calf serum (FCS) affects the development of bovine embryos in vitro.
- They found that using FCS led to earlier blastocyst formation and a higher number of cells compared to cultures without any supplements.
- The study suggests that specific components in the serum accelerate embryo development, particularly between crucial cell division stages.
Establishment of an embryonic stem cell line from 8-cell stage mouse embryos.
Eur J Morphol
November 1996
The aim of this study was to try establishing mouse ES cell lines from the early developmental stage. Fifty-two uncompacted 8-cell stage embryos were dissociated and single blastomeres were seeded on primary embryonic fibroblasts in DMEM/F12 completed with 10% foetal calf serum, 10% new born calf serum. 10(-4) M beta-mercaptoethanol.
View Article and Find Full Text PDF