NTRK fusion events and targeted treatment of advanced radioiodine refractory thyroid cancer.

Purpose: Pathogenic fusion events involving neurotrophic receptor tyrosine kinase (NTRK) have been described in ~ 2% of differentiated thyroid cancer (DTC). The selective tropomyosin receptor kinase (TRK) inhibitors entrectinib and larotrectinib have been approved in a tumor agnostic manner based on phase 1/2 clinical trials. In a real-world setting at five referral centers, we aimed to describe the prevalence of NTRK gene fusions and the efficacy and safety of TRK inhibitor treatment for non-medullary, advanced thyroid cancer (TC).

Stereo- and regioselective azide/alkyne cycloadditions in carbonic anhydrase II via tethering, monitored by crystallography and mass spectrometry.

The carbonic anhydrase II mutant His64Cys was prepared and applied to tethered alkyne/azide cycloaddition reactions. The azide component could be tethered to the enzyme surface through a disulfide bridge, while the alkyne component was reversibly coordinated through a sulfonamide anchor to the zinc ion in the original catalytic center of the enzyme. The incipient orientation of the reactants in the binding site and of the formed triazole product were characterized by crystallography.

[The acid-base equilibrium and carbohydrate metabolism during infection with Eperythrozoon suis].

Following on from clinical observations which point to severe metabolic disturbances in association with acute Eperythrozoon (E.) suis infection, the parameters of acid-base balance (pO2, pCO2, pH, actual bicarbonate, standard bicarbonate, base excess) as well as the glucose-, lactate- and pyruvate levels, were measured in venous blood during the course of eperythrozoonotic infection. Glucose consumption was investigated in in vitro experiments with differing numbers of pathogens.

[The effect of glucocorticoids on glucose metabolism in pygmy goats. 2. Glucose turnover and recycling].

In the present study glucose kinetic parameters like pool size, distribution volume and rates of total entry and irreversible loss were determined in four castrated male African pygmy goats during hyperglycemia induced by an intravenous glucocorticoid injection (0.25 mg dexamethasone = DXM per kg body weight). Experiments were done at two different metabolic states, "fed" once daily and "fasted" after three days of starvation.

[The effect of glucocorticoid on the glucose metabolism of pigmy goats. 1. Selected metabolites of energy metabolism].

In order to study mechanisms in ruminants responsible for glucocorticoid-induced hyperglycemia the effects of a single intravenous dose of a glucocorticoid on energy metabolism were investigated by measuring plasma levels of glucose, lactate, free fatty acids (FFA), 3-hydroxybutyrate (3-HOB) and acetoacetate (AcAc). Experiments were performed on four castrated male African pygmy goats at two metabolic states: "fed" with a single daily meal of hey and "fasted" with starvation for 3 days. The untreated goats served as their own control.