Bio-functionalized silver nanoparticles for selective colorimetric sensing of toxic metal ions and antimicrobial studies.

Hibiscus Sabdariffa (Gongura) plant extracts (leaves (HL) and stem (HS)) were used for the first time in the green synthesis of bio-functionalized silver nanoparticles (AgNPs). The bio-functionality of AgNPs has been successfully utilized for selective colorimetric sensing of potentially health and environmentally hazardous Hg(2+), Cd(2+) and Pb(2+) metal ions at ppm level in aqueous solution. Importantly, clearly distinguishable colour for all three metal ions was observed.

Green synthesized silver nanoparticles for selective colorimetric sensing of Hg2+ in aqueous solution at wide pH range.

In the present study, the syntheses of crystalline silver and gold nanoparticles (NPs) has been demonstrated in a green and environmentally friendly approach using citrus fruit extracts (lemon, Citrus limon (Cl-1) and sweet orange, Citrus limetta (Cl-2)). In addition, potentially hazardous metal ion sensing properties of these NPs in aqueous solution has been explored. Cl-1 and Cl-2 that predominantly contained citric and ascorbic acid, exhibited different reducing abilities towards silver and gold ions into NPs.

Two step purification of Acinetobacter sp. lipase and its evaluation as a detergent additive at low temperatures.

Acinetobacter sp. lipase was purified to homogeneity by a two-step process. The crude enzyme (along with biomass) was subjected to partial purification by aqueous two phase system (ATPS), avoiding centrifugation and filtration steps.

Efficacy of lipase from Aspergillus niger as an additive in detergent formulations: a statistical approach.

The efficacy of lipase from Aspergillus niger MTCC 2594 as an additive in laundry detergent formulations was assessed using response surface methodology (RSM). A five-level four-factorial central composite design was chosen to explain the washing protocol with four critical factors, viz. detergent concentration, lipase concentration, buffer pH and washing temperature.