[Role of gene polymorphisms of phase II of xenobiotic biotransformation from glutathione-S-transferase and N-acetyltransferase families in susceptibility to lung cancer among Mayak workers].

An association between polymorphous (allelic) gene variants of phase II of enzymatic xenobiotic biotransformation (EXB) of multigene families of glutathione-S-transferase (GSTs) GSTM1*0, GSTT1*0, GSTP1*B Ile105Val, and N-acetyltransferase (NAT) NAT2*6 590G>A, NAT2*5 481C>T, as well as lung cancer in Mayak workers exposed occupationally to prolonged external γ-rays and internal α-radiation from incorporated 239Pu was studied. Analysis of the population frequency of genotypes and alleles of the studied genes in the cohort of Mayak workers revealed their compliance with the Hardy-Weinberg principle and with the corresponding frequency in the European population. The study was based on the case-control method.

[Analysis of genome instability in offspring of "Mayak" workers families: minisatellite CEB1].

Genome instability transmission in offspring was analyzed in order to evaluate the risk of delayed genetic effects of exposure in 95 family triplets in which only fathers experienced prolonged occupational radiation exposure. The mean total preconceptive absorbed dose (TPAD) of external gamma radiation in the paternal gonads was 1.65 ± 0.

[Possibility of DNA isolation from archived autopsy tissues for molecular genetic studies].

Optimal conditions were defined for DNA isolation from formalin-fixed and paraffin-embedded (FFPE) archived autopsy lung tissue slices. The quality of DNA preparations isolated from the FFPE archived slices was assessed and their suitability for further molecular genetic analysis estimated. DNA isolated from the FFPE slices stored less and more than 10 years was suitable for molecular genetic studies in 100 and 66.

[Role of ancestral genotypes of the genes of xenobiotics biotransformation in susceptibility to lung cancer among Mayak workers].

An association between polymorphous (allelic) variants of genes of the 1st Phase of enzymatic xenobiotic biotransformation (EXB) in the superfamily of cytochromes P450 gene CYP1A1 * 2A (ml polymorphism), CYP1A1 * 2C (m2 polymorphism), gene CYP2E1 * 6 (C polymorphism), and between polymorphous vari- ants of gene of the 2nd EXB Phase of mircosomal epoxide hydrolase mEPOX Tyr113His (EH3 polymor- phism), and mEPOXHis139Arg (EH4 polymorphism) and lung cancer in Mayak workers with occupational prolonged exposure was studied. Analysis of population genotype frequency and alleles of genes CYP1A1 * 2A, CYP1A1 * 2C, CYP2E1 * 6, mEPOX Tyr113His, and mEPOXHis139Arg was conducted in the group of Mayak workers (289 individuals). The study has shown their compliance with the Hardy-Weinberg equilibrium, and correspondence of the genotype frequency to their frequency in European population.