Prokaryote- and Eukaryote-Based Expression Systems: Advances in Post-Pandemic Viral Antigen Production for Vaccines.

This review addresses the ongoing global challenge posed by emerging and evolving viral diseases, underscoring the need for innovative vaccine development strategies. It focuses on the modern approaches to creating vaccines based on recombinant proteins produced in different expression systems, including bacteria, yeast, plants, insects, and mammals. This review analyses the advantages, limitations, and applications of these expression systems for producing vaccine antigens, as well as strategies for designing safer, more effective, and potentially 'universal' antigens.

[Rats' reproductive function under toxic load against the background of protein deficiency].

Safety assessment approaches of novel food, currently adopted in the Russian Federation, imply mandatory in vivo reproductive toxicity tests with the study of reproductive function and offspring development in order to obtain comprehensive evidence of the absence of distant adverse effects that may manifest only in the next generation. Comprehensive study of reproductive function, pre- and postnatal offspring development, as a rule, includes the research of a large number of parameters, all of which has a wide range of physiological fluctuations, and the heterogeneous distribution of some parameters' values complicates the interpretation of the results. of the study was to investigate the efficiency of a reduced adaptive potential model in rats, based on decreased diet protein intake, for the use in reproductive toxicity experiments.

Mechanical properties variation of samples fabricated by fused deposition additive manufacturing as a function of filler percentage and structure for different plastics.

One of the key factors in manufacturing products by fused deposition molding (FDM) or layer-by-layer printing technology is the material intensity of the product. The task of reducing the amount of material required to manufacture a product without significant loss of mechanical properties is one of the most practically important technological tasks. Material saving in FDM printing of products allows to reduce financial costs and increase the speed of manufacturing of the final product without reducing (or not significantly reducing) the quality properties of the product.

Assessment of a Structurally Modified Alternanthera Mosaic Plant Virus as a Delivery System for Sarcoma Cells.

The virions of plant viruses and their structurally modified particles (SP) represent valuable platforms for recombinant vaccine epitopes and antitumor agents. The possibility of modifying their surface with biological compounds makes them a tool for developing medical biotechnology applications. Here, we applied a new type of SP derived from virions and virus-like particles (VLP) of Alternanthera mosaic virus (AltMV) and well-studied SP from Tobacco mosaic virus (TMV).