[A comparative study of liposome-incorporated beta-lactam antibiotics, fluoroquinolones and cephalosporin conjugates with fluoroquinolones].

Beta-lactam antibiotics (some penicillins and cephalosporins), a fluorquinolone (pefloxacin) and cephalosporin conjugates with fluorquinolones were liposome-encapsulated by the method of detergent dialysis. Under the experimental conditions the entrapment of the beta-lactams and pefloxacin to liposomes did not exceed 20 and 12.5 per cent of the initial quantity respectively.

[Inactivation of free and liposome-encapsulated aminoglycosides by enzymes of resistant strains of bacteria].

Liposome-encapsulated gentamicin, sisomicin, paromomycin, amikacin, dibekacin and some other aminoglycoside antibiotics were prepared by the method of detergent dialysis. The entrapment level was higher with respect to paromomycin, gentamicin and sisomicin. After a 1-hour incubation of the liposome-encapsulated paromomycin in the presence of a cell-free preparation of 3'-1-phosphotransferase from Escherichia coli there was observed no inactivation of the antibiotic.

[Obtaining liposomal preparations of various biologically active substances by the method of detergent flow analysis].

It was shown that detergent dialysis could be successfully used for liposomal encapsulation of substances belonging to different chemical groups with diverse therapeutic activity such as rifampicin, aclarubicin, amphotericin B, pefloxacin and insulin. Liposome encapsulation of substances poorly soluble or insoluble in aqueous media was likely the most promising. The optimal incorporation depended on both the composition of the lipids forming the liposomes and the properties of the compounds being encapsulated.

[Incorporation of an antineoplastic drug aclarubicin into liposomes in relation to the conditions of its encapsulation].

The results of liposome drug encapsulation of aclarubicin (aclacinomycin A), an antitumor antibiotic, are presented. The method of flow detergent dialysis was applied. Conditions providing maximum encapsulation of aclarubicin (the ratio of lipid components and the lipid/detergent ratio), as well as conditions providing stability of liposomal emulsion (the presence of antioxidants, stearic acid and cholesterol) were defined.

[Ultrastructure of liposomes (interliposomal bonds)].

Interliposomal bonds (ILBS) analogous to intercellular bonds (ICBs) in microbial cultures were detected by electron microscopy in the liposomal materials obtained after encapsulation of substances of various chemical structure. Possible nonspecific formation of the bonds between biological membrane-limited objects (ILBs and ICBs) was suggested and formation of such bonds in liposome encapsulated drugs was believed to be of importance.