Publications by authors named "N Ravida"

Article Synopsis
  • - Current guidelines suggest observing anaphylactic patients for 4-6 hours after receiving epinephrine, but there's conflicting evidence about its effectiveness, especially for children.
  • - This study looked at pediatric patients treated for anaphylaxis in a single ED from 2017-2022 to see how often biphasic reactions occurred and if the observation periods were adequate.
  • - Out of 292 patients observed for an average of about 233 minutes, only 3.4% developed biphasic reactions, with no significant differences found in observation duration or timing of epinephrine administration between those who did and did not experience these reactions.
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Context: With two northern white rhinos (NWR) remaining, the continued existence of this species relies on studying their relative, the southern white rhino (SWR).

Aims: (1) Characterise gene expression in granulosa cells (GC) from SWR cumulus oocyte complexes (COCs) prior to (Pre-) and after (Post-) in vitro maturation (IVM), comparing culture media and oocytes from donors treated with or without gonadotropin stimulation prior to ovum recovery; and (2) evaluate COC glucose consumption in spent media.

Methods: COCs were retrieved from four SWRs.

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Burmese pythons Python bivittatus captured in the Florida Everglades as part of an invasive species monitoring program served as a model for the development of sperm cryopreservation protocols for endangered snakes. Spermatozoa were collected from the vas deferens and initial motility, plasma membrane integrity and acrosome integrity were recorded before cryopreservation. Spermatozoa were extended in TES and Tris (TEST) yolk buffer with glycerol (GLY) or dimethyl sulfoxide (DMSO) concentrations of 8%, 12% or 16%, or combinations of GLY and DMSO with final concentrations of 4%:4%, 6%:6% or 8%:8%, and frozen at a rate of 0.

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