Hydrophilic interaction chromatography coupled to high resolution mass spectrometry (HILIC-LC-HRMS): An approach to study natural peptides in Viperidae snake venom.

Although proteins in snake venoms have been extensively studied and characterized, low-mass molecules remain relatively unexplored, mainly due to their low abundance, secondary role in envenomation, and some analytical technique limitations. However, these small molecules can provide new important data related to venom toxins' molecular structure, functions, and evolutionary relationships. This research aimed to characterize molecules below 10 kDa in the venoms of snakes from the Viperidae families (Bothrops, Agkistrodon, and Bitis) and compare two chromatographic approaches: reverse-phase chromatography (RP), a classic technique, and hydrophilic interaction liquid chromatography (HILIC), an alternative technique, both coupled with high-resolution mass spectrometry (HRMS).

Encapsulated LyeTx III Peptide: Cytotoxic Agent Isolated from Spider Venom.

The discovery of novel cytotoxic drugs is of paramount importance in contemporary medical research, particularly in the search for treatments with fewer side effects and higher specificity. Antimicrobial peptides are an interesting class of molecules for this endeavor. In this context, the LyeTx III, a new peptide extracted from the venom of the spider, stands out.

Occurrence and molecular identification of haemotropic Mycoplasma species in grey wolves (Canis lupus) from southern Europe.

Although wild and domestic carnivores share some haemotropic Mycoplasma species, information about the circulation of this pathogen in grey wolves (Canis lupus) populations is still very limited. Thus, a geographically broad-based investigation was performed for determining the occurrence and diversity of Mycoplasma spp. in three different wolf populations from southern Europe.

Mapping of the trans population in the Baixada Santista region, Brazil, 2023: a descriptive study.

Objective: To describe the socioeconomic and demographic characteristics of the trans population in the Baixada Santista region, São Paulo state.

Methods: This was a descriptive study involving adult trans people, selected through convenience sampling in 2023. A quantitative questionnaire was administered and in-depth interviews were conducted, which were analyzed using thematic grouping.

Micro and macropolitical determinants for non-vaccination against COVID-19 in pregnant women in Belo Horizonte.

Objective: To analyze the determinants for non-vaccination against COVID-19 in pregnant women in Belo Horizonte, Minas Gerais, Brazil.

Methods: An epidemiological study with a cross-sectional design was conducted using data from the project titled "Childbirth and Breastfeeding in Children of Mothers Infected by SARS-CoV-2," developed during the pandemic in the city of Belo Horizonte, Minas Gerais, Brazil.

Results: The study sample consisted of 360 pregnant women, of whom 77.