Publications by authors named "N R Ratcliffe"
Article Synopsis
- This study focuses on creating and testing 6-aminated 1,4-benzoquinones (6-AQs) for their ability to combat seven resistant pathogens.
- The 6-AQs were synthesized using a reaction between bromoquinone and substituted anilines, and their antimicrobial effectiveness was analyzed, revealing minimum inhibitory concentrations between 16 to 128 μg/ml.
- Notably, two derivatives showed effectiveness against methicillin-resistant strains, showcasing both bacteriostatic and bactericidal properties and suggesting these compounds as potential new treatments.
Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes.
Dev Comp Immunol
October 2024
Consideration is given to previous and more recent protocols for harvesting arthropod haemocytes from Galleria, Drosophila, mosquitoes, Limulus and crustaceans. The optimal harvesting of these cells is essential for meaningful studies of invertebrate immunity in vitro. The results of such experiments, however, have often been flawed due to a lack of understanding of the fragile nature of arthropod haemocytes on exposure to bacterial lipopolysaccharides, resulting in the aggregation and loss of cell types during haemolymph clotting.
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- Importance of PPIE
- : Patient and public involvement and engagement (PPIE) are crucial for designing effective trials, as they enhance participant experience and overall delivery.
- EJS ACT-PD Initiative
- : The UK consortium aims to integrate PPIE into all aspects of a clinical trial focused on disease-modifying therapies for Parkinson's Disease (PD).
- Evaluation and Impact
- : A working group involving patients and care partners is developing processes to evaluate PPIE effectiveness, using various research methods to assess the quality and impact of their involvement on trial outcomes.
This overview initially describes insect immune reactions and then brings together present knowledge of the interactions of vector insects with their invading parasites and pathogens. It is a way of introducing this Special Issue with subsequent papers presenting the latest details of these interactions in each particular group of vectors. Hopefully, this paper will fill a void in the literature since brief descriptions of vector immunity have now been brought together in one publication and could form a starting point for those interested and new to this important area.
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