Development of dairy herd of transgenic goats as biofactory for large-scale production of biologically active recombinant human lactoferrin.

The primary male-goats Lac-1 (human lactoferrin gene construct hLF5) and Lac-2 (human lactoferrin gene construct hLF3) with genome containing human lactoferrin gene were bred and the sperm bank of primary male-goats and their male descendents (F-F) was created. The herd of goats (200 transgenic females) that produced recombinant human lactoferrin (rhLF) in their milk at levels up to 16 g/L was obtained. The rhLF from milk of transgenic goats, natural human lactoferrin (hLF) from woman milk and natural goat lactoferrin (gLF) from milk of non-transgenic goats were purified using cation-exchange chromatography.

Plasma x-ray radiation source.

This paper gives the results of studies on a plasma x-ray source, which enables one to obtain a 2.5-krad radiation dose per pulse over an area of 100 cm2 in the quantum energy range from 20 to 500 keV. Pulse duration is 100 ns.

A 10-TW Pulsed Facility "PIRIT" for Investigation of Short-Wave Radiation Generation.

The results of experiments with a plasma x-ray source in the PIRIT-2000 facility are presented in this paper. The facility is designed with module capacitive energy storage energizing vacuum inductive storage. The formation of a rapidly growing current pulse as well as its commutation on a load was carried out by a plasma opening switch.

Plasma x-ray sources powered by megajoule magnetocumulative generators.

We have performed experiments using magnetocumulative generators (MCGs) to power three different types of high-energy-density plasma discharges suitable for intense x-ray generation. They include the H-pressed discharge, the capillary z-pinch, and the θ-pinch. The MCGs were operated with, and without, plasma opening switches.