Effects of vasoactive peptides on cytosolic calcium in cultured mesangial cells.

We have used the "second generation" Ca indicator, fura-2, to measure cytosolic free Ca concentrations in superfused cultures of adherent primary renal mesangial cells. The basal cytosolic free Ca concentration in these cells was found to be 93 +/- 5 nM (n = 35). The Ca ionophore ionomycin (0.

Bradykinin-evoked modulation of cytosolic Ca2+ concentrations in cultured renal epithelial (MDCK) cells.

We have investigated the effect of bradykinin on cytosolic Ca2+ concentrations of renal MDCK cells cultured as monolayers. Bradykinin rapidly (within 5 to 20 s) increased cytosolic Ca2+ concentrations, measured by using the fluorescent indicator quin-2, from the basal value of 103 nM to a maximal value of 578 nM at about 10(-8) M bradykinin. The increase of Ca2+ was transient, returning to baseline within 1.