[SPECIFIC IMMUNE RESPONSE TO CERTAIN CAPSULE POLYSACCHARIDES OF STREPTOCOCCUS PNEUMONIAE IN HEALTHY BLOOD DONORS AND INDIVIDUALS IMMUNIZED WITH PNEUMOCOCCAL VACCINES].

Aim: Evaluate humoral immune IgG response to 13 specific capsule polysaccharides in healthy blood donors and individuals immunized with pneumococcal vaccines.

Materials And Methods: A solid-phase EIA method was used with sorption on polystyrol of pneumococcal CPS isolated by us (1, 3, 4, 6A, 6B, 7F, 9N, 9V, 14, 18C, 19A, 19F, 23F). Blood sera of 140 healthy donors, 42 conscripts (young males) vaccinated with Pneumo-23 and 5 adults that are frequently ill with various bronchial-lung diseases for a long time and vaccinated with Prevenar-13 preparation were analyzed.

[Experience of application of pneumococcus vaccines for studies of humoral immunity].

Aim: Evaluate possible use of Pneumo-23 pneumococcus polysaccharide vaccine and Prevenar-7 conjugated vaccine in EIA as antigens for determination of IgG levels against capsule polysaccharides.

Materials And Methods: Solid phase EIA method with sorption on polystyrol of commercial vaccines Penumo-23 and Prevenar-7 was used in the study. Blood sera of 41 children before immunization and sera of 8 children before and after vaccination with Pneumo-23 and Prevenar-7 were analyzed.

[Study of cross-activity of Streptococcus pneumoniae antigen preparations].

Aim: Study cross-activity of S. pneumoniae antigen preparations.

Materials And Methods: Antigen preparations were obtained by ultrasound disintegration (from bacteria in R-form), extraction with water (from serotype 3 bacteria), cetavlon and trichloroacetic acid (from serotype 6A bacteria).

[Immunobiologic characteristics of carbohydrate-containing preparations of Haemophilus influenzae].

Aim: Comparative assessment of immunobiological characteristics of 3 antigenic preparations containing capsular polysaccharide of Haemophilus influenzae type b (CPS Hib).

Materials And Methods: The following preparations were assessed: CPS Hib obtained by using cetavlon; hydroxylamine preparation of Hib (HAP Hib); mixture of CPS Hib and lipooligosaccharide of non-typeable H. influenzae (LOS NTHi) detoxified by hydroxylamine hydrochloride.

[Subtyping of lipooligosaccharides of non-typeable haemophilus influenzae isolated from children with bronchopulmonary diseases].

Aim: Subtyping of lipooligosaccharides (LOS) of non-typeable strains of Haemophilus influenzae (NTHi) isolated from children with bronchopulmonary diseases.

Materials And Methods: Lipooligosaccharides obtained from 62 acapsular strains of H. influenzae were studied by vertical SDS-electrophoresis in PAAG.