Multiplexed inhibition of immunosuppressive genes with Cas13d for combinatorial cancer immunotherapy.

The complex nature of the immunosuppressive tumor microenvironment (TME) requires multi-agent combinations for optimal immunotherapy. Here we describe multiplex universal combinatorial immunotherapy via gene silencing (MUCIG), which uses CRISPR-Cas13d to silence multiple endogenous immunosuppressive genes in the TME, promoting TME remodeling and enhancing antitumor immunity. MUCIG vectors targeting four genes delivered by adeno-associated virus (AAV) (Cd274/Pdl1, Lgals9/Galectin9, Lgals3/Galectin3 and Cd47; AAV-Cas13d-PGGC) demonstrate significant antitumor efficacy across multiple syngeneic tumor models, remodeling the TME by increasing CD8 T-cell infiltration while reducing neutrophils.

Investigation of Spectroscopic Parameters and Trap Parameters of Eu-Activated YSiO Phosphors for Display and Dosimetry Applications.

Using the solid-state reaction technique, varied YSiO phosphors activated by europium (Eu) ions at varied concentrations were made at calcination temperatures of 1000 °C and 1250 °C during sintering in an air environment. The XRD technique identified the monoclinic structure, and the FTIR technique was used to analyze the generated phosphors. Photoluminescence emission and excitation patterns were measured using varying concentrations of Eu ions.

Comparing the Existence of Golden Proportion Using Maxillary Anterior Teeth Dimensions in Primary and Permanent Dentition: A Cross-sectional Study.

Aims And Background: The golden proportion is based on the premise that there is a link between natural beauty and mathematics. The study aimed to analyze the mesiodistal width of maxillary anterior teeth in primary and permanent dentition to determine whether a golden proportion exists among them.

Materials And Methods: Sixty subjects were randomly selected in accordance with inclusion and exclusion criteria.

Arthroscopic Transosseous-Equivalent Double-Row Rotator Cuff Repair Augmentation With Interpositional Demineralized Bone Fiber Implant.

Failure of rotator cuff repairs contributes to decreased patient satisfaction and quality of life. Biologic enhancement of repairs represents a novel augmentation strategy attempting to reproduce native healing while concomitantly potentially decreasing the existing high failure rates associated with rotator cuff repairs. Scaffolds placed on top of the rotator cuff have been widely studied, yet no recreation of the native enthesis is achieved via this augmentation strategy.