Publications by authors named "N P Chernysheva"
Article Synopsis
- Researchers explored the reaction of red phosphorus with alkyl bromides using strong bases and phase transfer catalysts to create sulfur-containing organophosphorus compounds that function as extractants for heavy metals.
- The reaction conditions involved heating the mixture and then adding elemental sulfur, resulting in a range of products, primarily alkylphosphines and their oxides.
- Testing revealed that the synthesized mixtures of alkylphosphine sulfides and oxides effectively extract heavy metals like Ni, Co, Zn, Pb, and noble metals such as Ag, achieving extraction rates of over 99%.
Derivatives of natural allylpolyalkoxybenzenes conjugated to triphenylphosphonium (TPP) cations by aliphatic linkers of three, six, seven, and eight atoms were synthesized to examine the role of the polyalkoxybenzene pharmacophore, TPP fragment, and linker length in antiproliferative activities. The key synthetic procedures included (i) hydroboration-oxidation of apiol, dillapiol, myristicin, and allyltetramethoxybenzene; (ii) acylation of polyalkoxybenzyl alcohols or amines; and (iii) condensation of polyalkoxybenzaldehydes followed by hydrogenation and cyclopropyl-homoallyl rearrangement. The targeted TPP conjugates as well as the starting allylbenzenes, the corresponding alkylpolyalkoxybenzenes, and the respective alkyl-TPP salts were evaluated for cytotoxicity in a panel of human cancer cell lines using MTT and Click-iT-EdU assays and in a sea urchin embryo model.
View Article and Find Full Text PDFHere, we investigated general porin regulation in 488, the causative agent of Far Eastern scarlet-like fever, in response to sublethal concentrations of antibiotics. We chose four antibiotics of different classes and measured gene expression using qRT-PCR and GFP reporter systems. Our data showed temporal regulation of the general porin genes and caused by antibiotic stress.
View Article and Find Full Text PDFA Gram-negative, non-motile bacterium КMM 3653 was isolated from a sediment sample from the Sea of Japan seashore, Russia. On the basis of the 16S rRNA gene sequence analysis the strain КMM 3653 was positioned within the family Rhodobacteraceae (class Alphaproteobacteria) forming a distinct lineage with the highest gene sequence similarities to the members of the genera Pacificibacter (95.2-94.
View Article and Find Full Text PDFWe carried out a detailed investigation of PL7 alginate lyases across the genus. The main findings were obtained using the methods of comparative genomics and spatial structure modeling, as well as a phylogenomic approach. Initially, in order to elucidate the alginolytic potential of , we calculated the content of polysaccharide lyase (PL) genes in each genome.
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