Publications by authors named "N Nino-Martinez"

Background:  In large-scale molecular studies, a protocol that generates high yields and quality DNA for future polymerase chain reaction (PCR) assays is needed. The collection of buccal cells by cytobrush may represent an efficient, noninvasive, and inexpensive method for obtaining genetic material from school populations. The aim of this study was to develop a method to obtain genomic DNA from buccal cells of schoolchildren, and the DNA was extracted immediately after collecting the buccal cell samples and after storing the samples for 8 months at -20 °C to establish the feasibility of the method for epidemiological studies.

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Article Synopsis
  • The study assessed the effectiveness of bioceramic sealers, specifically CeraSeal, EndoSequence BC Sealer, and Bio-C Sealer, supplemented with silver nanoparticles (AgNPs) in terms of push-out bond strength, surface roughness, and antimicrobial properties.
  • No significant differences in bond strength were found between sealers with and without AgNPs in most areas tested, although EndoSequence BC Sealer showed a stronger bond in the middle third.
  • All sealers inhibited bacterial growth, with EndoSequence BC Sealer demonstrating the highest antimicrobial activity, indicating that adding AgNPs enhances antimicrobial properties without compromising mechanical strength.
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Bone is a natural nanocomposite composed of proteins and minerals that can regenerate itself. However, there are conditions in which this process is impaired, such as extensive bone defects and infections of the bone or surrounding tissue. This study evaluates the osteoregenerative capacity of bone grafting materials in animals with induced bone defects.

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  • The study investigates the impact of orthodontic treatment on bacterial populations in the mouth, which can lead to an increased risk of white spot lesions caused by bacteria like Streptococcus mutans and Lactobacilli.
  • It explores the use of silver nanoparticles (AgNPs) added to three types of commercial orthodontic adhesives, measuring their effects on shear bond strength and bacterial resistance.
  • Results show that adding AgNPs does not affect the bond strength of the adhesives while enhancing their ability to kill harmful bacteria, making them a viable option for improving oral health during orthodontic treatment.
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Medical devices with antimicrobial properties are a potential long-term solution to the high rate of multi-drug-resistant healthcare-associated infections. Silver nanoparticles (AgNPs) are an established agent for effectively eliminating a wide range of microbial strains. AgNPs have been commonly incorporated into traditional plastic materials; however, recently, there has been increased interest in using AgNPs combined with 3D-printing technology for medical devices due to the accessibility and customizability of 3D-printed products.

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