Complete genome and proteome of Acholeplasma laidlawii.

We present the complete genome sequence and proteogenomic map for Acholeplasma laidlawii PG-8A (class Mollicutes, order Acholeplasmatales, family Acholeplasmataceae). The genome of A. laidlawii is represented by a single 1,496,992-bp circular chromosome with an average G+C content of 31 mol%.

The effect of three-dimensional structure on the solid state isotope exchange of hydrogen in polypeptides with spillover hydrogen.

The effect of the three-dimensional structure of polypeptides and proteins on their ability to undergo isotopic exchange under the action of spillover hydrogen (SH) in the high temperature solid state catalytic isotope exchange reaction (HSCIE) was theoretically and experimentally studied. The HSCIE reaction in the beta-galactosidase protein from Thermoanaerobacter ethanolicus (83kDa) was studied. The influence of the beta-galactosidase structure on isotopic exchange as peptide fragments with spillover tritium was studied.

Packing constraints of hydrophobic side chains in (alpha/beta)8 barrels.

An analysis of possible tight packing of hydrophobic groups simultaneously at the both surfaces of beta-hyperboloid-8 was conducted. This analysis shows that the disposition of amino acid side chains at the real beta-structure's surface is unique. If we sign the mean distance between adjacent beta-strands as "a," and the mean distance along beta-strand between C alpha atoms, whose side chains are directed to one side of the beta-sheet, as "b," the ratio b/a = square root of 2 very precisely.

The role of local tight packing of hydrophobic groups in beta-structure.

An analysis of the tendency of hydrophobic groups to tight packing on the surface of beta-sheets based on well-known parameters of beta-sheets and hydrophobic groups was conducted. This analysis shows the existence of very limited numbers and clearly outlined architecture families of regular parts for the majority of beta-structure-containing domains. Each family of architecture strongly depends on the number of beta-strands in the pure beta-domains and on the existence and number of additional alpha-helixes and on the mutual arrangements beta-strands and alpha-helixes along the chain in mixed alpha/beta-domains.