Complex formation with protamine prolongs the thrombin-inhibiting effect of DNA aptamer in vivo.

Antithrombin DNA aptamersRE31 are single-chain oligonucleotides that fold into three-dimensional forms allowing them to bind the enzyme with high affinity and inhibit its activity in vivo. They are rapidly degraded by a nonspecific nuclease, and, to prolong the lifetime of the aptamer DNA in the bloodstream, it is necessary to coat it with a polymer envelope. A new approach to solving this problem based on preparation of DNA-polyelectrolyte complexes with a minimal particle size that can circulate with blood flow.

[Age and sex features of antioxidation protection and free-radical processes in white rats brain].

With the years, the activity of antioxidation enzymes and speeds of its free-radical oxidation in different molecules changes. These changes depend also on an animal sex. The brain tissue is especially subjects to oxidising destruction and has the features of antioxidation protection.

[Several aspects of clinical signs and pathogenesis of pneumopathies in acute poisoning caused by sulfur-containing natural gas].

The article summarizes and analyzes data on 32 cases of acute intoxication varying in severity and caused by inhalation of sulfur-containing natural gas of Astrakhan gas deposit (SCNGAGD). The data were obtained in experimental poisoning of 120 Wistar rats with the gas. Pneumopathies (toxic pulmonary edema, pneumonitis, asthmatic bronchitis, alveolitis) appeared to play the central role among other syndromes of the intoxication by SCNGAGD.

[Changes in respiration and blood circulation during the inhalation of air mixtures with lethal and sublethal concentrations of hydrogen sulfide-containing natural gas].

Experiments in pentobarbital-anesthetized cats of both sexes showed that upon spontaneous inhalation of hydrogen sulphide-containing gas mixtures (600 mg/m3) respiratory and circulatory changes occur within 10-15 min (30% of animals) and continued to 20-25 min (100% of animals) leading to severe respiratory disturbances (apneusis, gasping) abrupt decrease of mean systemic arterial pressure and decreased cardiac output, i.e., the changes of the type of peripheral collapse.