Publications by authors named "N N Shirokova"
Article Synopsis
- - Connexin-43 (Cx43) is a key protein in heart cells that forms gap junctions, and its remodeling is connected to arrhythmias in heart issues like hypertrophy and heart failure.
- - In a study using a genetically modified mouse model, researchers found that opening of remodeled Cx43 can lead to severe arrhythmias during stress, even without other cardiac dysfunction.
- - The use of Cx43 hemichannel blockers demonstrated that these remodeled channels increase membrane permeability and cause calcium overload, leading to prolonged action potentials and triggering abnormal heart rhythms.
Article Synopsis
- Dilated cardiomyopathy is a major cause of death in Duchenne muscular dystrophy (DMD) due to the absence of the dystrophin protein, leading to disorganization of heart muscle cells and gap junctions.
- Research shows that the phosphorylation of connexin-43 (Cx43) is crucial for its proper function and localization, and that microtubule (MT) organization affects this process.
- Treatment with colchicine improved the structure of MTs and reduced harmful heart remodeling in DMD mice, highlighting the need for understanding pS-Cx43 and MT interactions to create new therapies for DMD-related heart issues.
Childhood dilated cardiomyopathy (DCM) is a leading cause of heart failure requiring cardiac transplantation and approximately 5% of cases result in sudden death. Knowledge of the underlying genetic cause can aid prognostication and clinical management and enables accurate recurrence risk counselling for the family. Here we used genomic sequencing to identify the causative genetic variant(s) in families with children affected by severe DCM.
View Article and Find Full Text PDFObjective: This study aims to investigate the callipyge gene () polymorphism in sheep of Edilbay, Volgograd, and Kalmyk breeds.
Materials And Methods: The analysis was performed by the polymerase chain reaction-restriction fragment length polymorphisms method. The objects of the study were Edilbay fat-tailed sheep ( = 500) at the breeding plant Volgograd-Edilbay (Volgograd region), Volgograd fine-wool sheep ( = 500) at the breeding plant Romashkovskiy (Volgograd region), and Kalmyk fat-tailed sheep ( = 500) at the breeding plant Kirovsky (the Republic of Kalmykia, Yashkul rayon).
Aberrant expression of the cardiac gap junction protein connexin-43 (Cx43) has been suggested as playing a role in the development of cardiac disease in the mdx mouse model of Duchenne muscular dystrophy (DMD); however, a mechanistic understanding of this association is lacking. Here, we identified a reduction of phosphorylation of Cx43 serines S325/S328/S330 in human and mouse DMD hearts. We hypothesized that hypophosphorylation of Cx43 serine-triplet triggers pathological Cx43 redistribution to the lateral sides of cardiomyocytes (remodeling).
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