Sources of Reactive Oxygen and Nitrogen Species in Tissue Microenvironment of Hernioplasty Materials.

Titanium and polypropylene mesh endoprostheses were implanted into the rat abdominal cavity and the populations of cells migrating to their surface were analyzed. On both materials, the cells were presented mainly by macrophages that proliferated, activated, and fused to form multinuclear cells. Contact with the foreign surface triggered superoxide anion generation and myeloperoxidase synthesis by macrophages (these processes were more intense on polypropylene implants than on titanium ones) and stimulated production of nitric oxide by macrophage.

[Modification of the method of trypsin esterase activity determination].

Determination of esterase activity of trypsin in blood serum using the Erlanger's method leads to overestimation of enzyme activity due to the increased "dimness" of the reaction medium. V. A.

Generation of free oxygen radicals in the pathogenesis of experimental acute reflux pancreatitis.

In dogs with acute reflux pancreatitis specific activity of superoxide dismutase in the pancreas rapidly increased (over 15 min). Activation of this enzyme by free oxygen radicals was demonstrated in in vitro experiments. Treatment with allopurinol prevented activation of superoxide dismutase.

Free radical oxidation and activity of enzymes catalyzing biotransformation of xenobiotics in the liver of rats with experimental acute pancreatitis.

The development of experimental acute pancreatitis in rats was accompanied by intensification of lipid peroxidation, activation of superoxide dismutase and glutathione reductase, inhibition of glutathione transferase, and deceleration of aniline N-hydroxylation by cytochrome P-450 in the liver. Antioxidants inhibited lipid peroxidation and antioxidant enzymes, but did not prevent inhibition of detoxifying enzymes.

[The level of malone dialdehyde in blood plasma as a manifestation of hepatic function in pilots of various professional categories].

Plasma levels of malone dialdehyde (MDA) were measured in flyers of various professional categories and persons who had no flight experience in normal conditions and shortly after a hypobaric hypoxia trial (30 min., P = 405 mm Hg, ascent and descent at 15-20 m/s), and after 24 hours of reoxygenation. In essentially healthy and highly adapted to periodic hypoxia flyers of the military transport aviation MDA remained within the normal range whereas in some subjects without flight experience displayed a significant increase in MDA levels during hypoxia and/or reoxygenation.