[The experimental treatment of cyanide poisoning with a methemoglobin solution].

The stroma-free methemoglobin solution proved to be an effective antidote against acute cyanide poisoning in experiment. The poisoning was induced by intraperitoneal administration to rats of cyanide solutions in doses of 5, 10 and 15 mg/kg. Methemoglobin solutions were injected intravenously in doses of 2 and 4 g/kg.

[The effect of the degree of hemoglobin polymerization on its interaction with the body].

Solutions of pyridoxylated polyhemoglobin (PPHb) with the different degree of polymerization (0-53%) were injected intravenously into rats (1 g/kg). The quantity of PPHb, circulating in plasma, being excreted with the urine and accumulated in the organs have been estimated during the first 6 hours after the infusion. During this period 25-40% of the injected PPHb have been found in the urine and 10-25% have been found in plasma, the percentage depending on the degree of polymerization.

[Biochemical properties of intramolecularly cross-linked hemoglobin].

The chemical modification of hemoglobin was conducted with the help of bifunctional crosslinking agent--glutaraldehyde. By SDS-polyacrylamide gel electrophoresis and gel-filtration it was shown that the final product contained 70% of modified protein which consisted of non-dissociating hemoglobin dimers and tetramers. It was also shown that the chemical modification didn't produce significant changes in the oxygen-transporting properties of the starting hemoglobin, but had influence on the character of the interaction with the allosteric regulator of reversible oxygenation (pyridoxal-5'-phosphate).

[Preservation of an isolated kidney in normothermic conditions by perfluorotributylamine emulsion perfusion].

Perfluorotributylamine emulsion (PFE) was used to preserve an isolated dog kidney under normothermal conditions with the aid of perfusion. The latter was carried out for 4 hours with the use of a pulse pump. The protein-saline solution (PSS) - 5% donor's albumin in Hanks solution, was used for reference purposes.