[Wide range of the use of natural lipases and esterases to inhibit Mycobacterium tuberculosis].

Lipases and/or esterases (hereinafter referred to as esterases) isolated from the wax moth (Galleria mellonella) were found to have a bacteriological action on Mycobacterium tuberculosis (MBT) H37Rv. Different types of raw esterase preparations (REP) were incubated with MBT at 37 degrees C for 18 hours, the incubate was seeded on the Finn-II solid medium or intraperitoneally injected into guinea pigs in a single dose of 100,000,000 bacteria. There was no growth of MBT in the medium within 8 weeks, some variants of REP causing a destruction of the medium for 3-7 days.

[Cross reactivity and amino acid structural homology of immunodominant peptide fragments of structural viruses from the human retroviruses HTLV-I, HTLV-II, HIV-1, and HIV-2].

Twelve synthetic peptides corresponding to 9 immunodominant regions of structural proteins of human retroviruses HTLV-I, HIV-1, and HIV-2 were studied in enzyme-linked immunosorbent assay for cross reactivity with heterotypical for each peptide anti-retroviral antibodies. The search of amino acid homologies was carried using the special computer program followed by the correspondence analysis of the discovered homologies and immunodominant fragments. It was found that peptides 100-130 p19 gag HTLV-I, 376-392 gp21 env HTLV-I, 381-400 gp21 env HTLV-I, 306-328 gp120 env HIV-1, 495-516 gp120 env HIV-1, 584-612 gp41 env HIV-1, and 581-603 gp36 env HIV-2 have type-specific reactivity and also cross react with 3-54% human sera containing antibodies against heterotypical retroviruses.

[Immunoreactivity of synthetic peptides, corresponding to B-cellular epitopes of human type I T-lymphotrophic virus structural proteins].

The immunoreactivity of 25 synthetic peptides corresponding to amino acid fragments of the HTLV-I structural proteins p19 gag, gp46 and gp21 env were studied in enzyme linked immunosorbent assay using a serum panel of 70 reference positive specimens with anti-HTLV-I antibodies. The location of the synthetic peptides containing the B-cell epitopes of HTLV-I was established. Anti-HTLV-I antibodies effectively recognized these peptides.

[A synthetic peptide-based immunoenzyme test system for the joint detection of human retrovirus (HTLV-1 and HIV-1) antibodies].

The study was made to develop an immunodiagnostic test system based on synthetic peptides able to detect in the same assay the total concentration of heterospecific antibodies against human retroviruses HTLV-1 and HIV-1. Three panels of reference-sera contained antibodies to HTLV-1 (70 specimens), HIV-1 (50 and 16 specimens) and 4 synthetic peptides corresponding to protein fragments p19 gag and gp46 env HTLV-1, gp120 and gp41 env HIV-1. Immune reactivity of the peptides with reference sera was measured in the immunoassay.