Publications by authors named "N N Ermakova"
We studied the effect of reprogrammed CD8 T cells (rT cells) from the bone marrow of intact mice on tumor cells and neovasculogenesis in mice with orthotopic Lewis lung carcinoma (LLC). Reprogramming of T cells was carried out using a MEK inhibitor and a PD-1 blocker; the targeting of rT cells to tumor cells was achieved by preincubation with LLC cell lysate. It was shown that the antitumor effect of rT cells was based on apoptosis of tumor cells.
View Article and Find Full Text PDFBackground: Awareness of age-related features of carcinogenesis and the importance of cellular immunity is crucial for developing effective antitumor therapies for specific patient groups.
Methods: In this study, we examined different populations of cancer stem cells (CSCs) and circulating tumor cells (CTCs) in "young" (8-10 weeks) and "aged" (80-82 weeks) C57BL/6 male mice. We used an orthotopic model of Lewis lung carcinoma (LLC) to evaluate the effectiveness of cell therapy targeting lung cancer through reprogrammed CD8-positive T cells (rCD8+ T cells) in mice from two different ages.
Article Synopsis
- The study examined the effects of the dopamine D2 receptor antagonist spiperone on combined lung conditions—emphysema and lung cancer—in C57BL/6 mice.
- Results indicated that spiperone reduced lung inflammation, tumor size, and metastasis, along with decreasing the number of cancer stem cells (CSCs) in both the lungs and blood of treated mice.
- The findings suggest that targeting dopamine D2 receptors could be a viable strategy for treating lung cancer in patients suffering from emphysema.
Background: Metastatic disease is a major and difficult-to-treat complication of lung cancer. Considering insufficient effectiveness of existing therapies and taking into account the current problem of lung cancer chemoresistance, it is necessary to continue the development of new treatments.
Methods: Previously, we have demonstrated the antitumor effects of reprogrammed CD8 T-cells (rCD8 T-cells) from the spleen in mice with orthotopic lung carcinoma.
The responses of tumor stem cells and various populations of CD4 and CD8 T cells of young and aged C57BL/6 mice were studied in a lung cancer model. Using Lewis lung carcinoma cell line, an orthotopic model of lung cancer was modeled. Cancer stem cells, circulating tumor cells, and various populations of CD4 and CD8 T cells in the blood and lung tissue were studied by cytometry.
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