Generation of functional islet-like clusters after monolayer culture and intracapsular aggregation of adult human pancreatic islet tissue.

Background: Cellular replacement therapy represents a promising strategy for treating type I diabetes; however, such an approach is limited due to the inadequate availability of human donor tissue. Here we investigated the extent to which human islet tissue can be expanded in monolayer culture and brought back to islet function.

Methods: Adult human pancreatic cells were proliferated with a serum-free media in monolayer cultures through multiple passages.

[Biocompatibility of microsurgical suture material modified with element-organic compounds].

By the method of tissue culture, it is established that the capron threads stained by brilliant green, coated with the chemosorbed substances on the base of titanium alkoxides, polyalkylhydridsiloxanes and polyorganilsiloxanes have no histotoxic properties and can be used in microsurgical practice.