Publications by authors named "N Manikanda Boopathi"
Deciphering gene specific molecular characterization and partial gene sequence for combined resistance to tomato leaf curl virus (ToLCV) (Ty-1, Ty-2 and Ty-3), fusarium wilt (I-2) and root- knot nematode (Mi-1) in selected fresh market breeding line of tomato (Solanum lycopersicom L.).
Mol Biol Rep
December 2024
Background: Tomato (Solanum lycopersicum L.) is a widely cultivated crop in tropical regions, but its production is often hampered by significant losses attributed to diseases like tomato leaf curl virus (ToLCV), fusarium wilt and root-knot nematode.
Methods And Results: This study employed an integrated approach utilizing both co-dominant and dominant SCAR markers, selected for specific resistance genes (ToLCV-Ty-1, Ty-2, Ty-2, Fusarium wilt (Race-2)-I-2, and Root-knot nematode-Mi-1.
Article Synopsis
- Surveys in Thanjavur and Coimbatore revealed up to 2.5% incidence, with unique symptoms that differ from previously known root wilt disease.
- New diagnostic methods like LAMP (Loop Mediated Isothermal Amplification) were developed for fast and accurate detection of phytoplasma, helping manage the disease and protect healthy coconut palms and seedlings.
This study was begun by establishing an culture in UPASI 9, a Nilgiris tea clone () by optimising various factors. Anatomical studies demonstrated that use of lower carbendazim concentration for sterilisation (0.2%) produced viable and healthy explants for callus initiation.
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- High production costs for conventional hybrid cotton seeds are driving research into cotton male sterility systems to enhance hybrid development.
- There is a significant gap in studies related to cytology, histology, and other factors needed to identify male sterility markers in cotton.
- The review highlights the importance of genetic male sterility (GMS) genes, particularly ms5ms6 in India, and discusses the use of molecular markers for effective backcrossing of male-sterility traits into elite cotton lines.
Background: Little leaf disease caused by phytoplasma infection is a significant threat to eggplant (also known as brinjal) cultivation in India. This study focused on the molecular characterisation of the phytoplasma strains and insect vectors responsible for its transmission and screening of brinjal germplasm for resistance to little leaf disease.
Results: Surveys conducted across districts in the Tamil Nadu state of India during 2021-2022 showed a higher incidence of phytoplasma during the Zaid (March to June), followed by Kharif (June to November) and Rabi (November to March) seasons with mean incidence ranging from 22 to 27%.