[Component activity of the isoenzyme spectra of Saccharomyces cerevisiae, Saccharomyces carlsbergensis and their hybrids].

The object of this work was to study the activity and the isozyme spectra of hexokinase (the triggering enzyme of glycolysis), glucose-6-phosphate dehydrogenase (the key enzyme of the pentose-phosphate shunt), malate dehydrogenase and isocitrate dehydrogenase (the enzymes of the citric acid cycle) and alcohol dehydrogenase (the enzyme involved in the first steps of ethanol oxidation) in Saccharomyces cerevisiae, race Ya, S. carlsbergensis, race 4228, and their hybrid 67. The parent organisms and their hybrid were shown to differ from one another in the qualitative composition and the activity of the isozyme spectra of the above enzymes.

[Comparative study of the spectra and activities of isoenzymes in haploid and autodiploid strains of Pichia pinus].

The spectra and activities of isozymes of hexokinase and alcohol dehydrogenase (enzymes that catalyse glucose and ethanol assimilation), glucose-6-phosphate dehydrogenase (the key enzyme of the pentose phosphate shunt) and malate dehydrogenase (an enzyme of the tricarboxylic acid cycle) were comparatively studied in Pichia pinus haploid (MH4) and autodiploid (D4) strains. Differences in the qualitative composition of the isoenzyme spectra and activities suggest that the intensity and the role of the studied metabolic pathways and cycles differ between the haploid and autodiploid strains of Pichia pinus.

[Comparative study of the physiological and biochemical characteristics of haploid and diploid Pichia pinus strains in their growth process on a glucose medium].

The economic coefficient, the growth rate, the quantitative content of bound amino acids, and the activities of hexokinase, pyruvate kinase, phosphorylase, malate dehydrogenase and glucose-6-phosphate dehydrogenase were studied comparatively in the haploid and diploid strains of Pichia pinus in the process of their growth in a medium containing glucose. An increase in the content of DNA in the cells was found to be accompanied with an increase of the specific growth rate at the exponential phase of growth and an increase in the activities of hexokinase and glucose-6-phosphate dehydrogenase; however, it had virtually no effect on the economic coefficient, the amino acid composition of protein, and the activities of pyruvate kinase and malate dehydrogenase.