[Use of neonatal induction of enzymes for the correction of experimental hereditary enzymopathies].

Administration of enzyme-inducing agents into newborn animals resulted in stable changes of the activities of the relevant inducible enzymes for long periods of their life in adulthood. The phenomenon designated as enzymic imprinting was used for correction of inherited enzymopathies in animals. Neonatal administration of galactose into the W/ssm rats with inherited galactosemia stably decreased galactose transport into the erythrocytes, increased activities of hexose oxidizing enzymes and prevented development of cataracts and other galactosemia symptoms.

[Effect of postnatal administration of a microsomal enzyme inducer on development of their activity during early mouse ontogeny].

The effect of early postnatal introduction of the inductor of microsomal enzymes, 3-acetate-16alpha-isothiocyanopregnenolone (ATCP), on the activity of cholesterol-7alpha-hydroxylase (cholesterol-reduced NADP+: oxygen oxydoreductase; 7alpha-hydroxylating) was studied. The experiments were carried out with newborn cholesteremic SWR/j mice which were given ATCP per os from the 2nd till the 9th day of postnatal development. The introduction of ATCP was shown to induce cholesterol-7alpha-hydroxylase in the liver of experimental mice during the first month of life.

Enzymic 'imprinting' as the result of early postnatal administration of enzyme inducers to animals.

Early postnatal treatment of rats or mice with different enzyme inducers (cortisol, galactose, steroid which induces mixed function oxidases) results in long-lasting changes in the activity of corresponding inducible enzymes.

[Effect of the early postnatal induction of microsomal enzymes on their activity and the cholesterol content in the blood of adult mice from a hypercholesteremic line].

Activity of microsomal enzymes and the patterns of cholesterol metabolism were studied in mice of WSR/y strain, characterized by spontaneous development of atherosclerosis within the later periods of life, after early postnatal administration of an inductor of the enzymes 3-acetate-16 alpha-isothiocyanopregnenolone (ATCP). Administration of ATCP into newborn mice of SWR/y strain, from the 2nd up to 16th day after birth, led to a stable increase in activity of arylhydrocarbonate hydroxylase (an enzyme participating in unspecific metabolism of drugs), which was observed during the whole experimental period (4 months). The treatment with ATCP caused also a distinct increase in activity of cholesterol-7 alpha-hydroxylase (a key enzyme of cholesterol biotransformation and elimination) as well as a considerable decrease in content of cholesterol and lipoprotein atherogenic fractions in blood serum.