Physicochemical properties and compatibility of sodium alginate and fucoidan solutions.

The interaction between linear sodium alginate and branched fucoidan were investigated using viscosity and tensiometric measurements. It was shown that water-soluble interpolymer complex is formed. The alginate-fucoidan complexation is due to the formation of a cooperative system of hydrogen bonds between the ionogenic and hidroxyl groups of sodium alginate and fucoidan and hydrophobic interactions.

Genome engineering of the Corynebacterium glutamicum chromosome by the Extended Dual-In/Out strategy.

A novel genome editing method for repeated introduction of foreign DNA, including insertion of rather large DNA fragments, into predesigned points in the Corynebacterium glutamicum chromosome was developed. The method is based on the implementation of the Dual-In/Out strategy, which was previously provided in Escherichia coli according to recombineering-based methods (Minaeva et al., 2008) and allowed step-by-step construction of marker-less plasmid free recombinant strains.

Immunosuppressive properties of cytochalasin B-induced membrane vesicles of mesenchymal stem cells: comparing with extracellular vesicles derived from mesenchymal stem cells.

Extracellular vesicles derived from mesenchymal stem cells (MSCs) represent a novel approach for regenerative and immunosuppressive therapy. Recently, cytochalasin B-induced microvesicles (CIMVs) were shown to be effective drug delivery mediators. However, little is known about their immunological properties.

Mu-driven transposition of recombinant mini-Mu unit DNA in the Corynebacterium glutamicum chromosome.

A dual-component Mu-transposition system was modified for the integration/amplification of genes in Corynebacterium. The system consists of two types of plasmids: (i) a non-replicative integrative plasmid that contains the transposing mini-Mu(LR) unit bracketed by the L/R Mu ends or the mini-Mu(LER) unit, which additionally contains the enhancer element, E, and (ii) an integration helper plasmid that expresses the transposition factor genes for MuA and MuB. Efficient transposition in the C.

[Biochemical indicators of blood at sportsmen at joint application of muscular and food loading].

The results of a blood biochemical testing among highly skilled sportsmen, coaching under different power conditions accompanied by veloergometer dosed stress and a trial carbohydrate breakfast are considered in the research. The quantity of glucose, crude protein, albumines, calcium, magnesium, potassium, chlorine and sodium were difined in the blood serum. Essential differences in the indices of glucose, magnesium, sodium, chlorine among skilled and non-skilled sportsmen were taped.