Severe cytomegalovirus disease with encephalitis after CAR-T cell therapy: A rare but potentially fatal complication.

Cytomegalovirus (CMV) is a ubiquitous herpes virus that develops lifelong latency following primary infection and can be reactivated following immune suppression. CMV encephalopathy has been described in few reports after hematopoietic stem cell transplantation and in patients with acquired immunodeficiency syndrome. To the best of our knowledge, CMV encephalopathy following CAR-T cells infusion had not been previously reported.

Electropolymerisable pyrrole-oligonucleotide: synthesis and analysis of ODN hybridisation by fluorescence and QCM.

Conducting polymer films, such as polypyrrole, appear particularly attractive for the immobilisation of biological molecules by entrapment or covalent grafting. We describe here a new pyrrole phosphorarnidite building block allowing the synthesis of oligonucleotide (ODN) bearing a pyrrole moiety. The electropolymerisable pyrrole moiety was then introduced on the 5' end of the oligonucleotide.

Polypyrrole based DNA hybridization assays: study of label free detection processes versus fluorescence on microchips.

In this paper, we present different ways to detect DNA hybridization on a solid support. The grafting chemistry is based on the electro-controlled copolymerization of a pyrrole-modified oligonucleotide and pyrrole. This process allows an easy functionalization of conducting materials.

Study of DNA hybridization on polypyrrole grafted with oligonucleotides by photocurrent spectroscopy.

Recognition of DNA sequences by biochemical sensor is generally performed by analysis after completion of hybridization. Using a technique able to directly translate the biological event into an electrical signal allows the in situ monitoring of the hybridization kinetics. In this aim, the photoelectrochemical behavior of one electroactive polymeric sensor based on a copolymer of polypyrrole and polypyrrole-oligonucleotide has been investigated in aqueous solution.