[Novel diagnostic kit on the basis of stained polymeric carriers for epidemiologic surveillance in natural foci of Crimean-Congo hemorrhagic fever].

Aim: To study diagnostic value of developed antigenic polymeric diagnostic kit for epidemiologic surveillance in natural foci of Crimean-Congo hemorrhagic fever (CCHF) in South federal district.

Materials And Methods: Novel antigenic diagnostic kit on the basis of polymeric microspheres for reaction of volume agglomeration was developed. The kit is designed for detection of virus-specific antibodies in human serum and in serum of agricultural animals.

[Genotypic heterogeneity and geographic diversity of collection strains of Francisella tularensis as determined using the VNTR variability analysis and DNA sequencing].

The analysis of the identification genotypes allowed the strains to be grouped into 61 variants from A to I with the incidence rate 0.002-0.142.

[Vntr-genotyping of Francisella tularensis strains isolated in the former USSR territory and some European countries during epizootics in 1988 - 1989].

Retrospective VNTR-analysis of 159 Francisella tularensis subsp. holarctica strains isolated in December 1988 - February 1989 in former USSR and some European countries was carried out. Analysis of heterogenic genotypes of strains allow to subdivide them into 30 groups of variants by individual genotypes, while cluster analysis--to subdivide them in 7 clusters with different number of compositions.

[Genotyping of the Francisella tularensis strains isolated from natural foci of tularemia in the Rostov region by multilocus VNTR analysis].

On the basis of an analysis of the VNTR alleles' distribution in 109 strains of F. tularensis it was established that 19 genotypes of the disease causative agent circulated in the Rostov Region from 1945 to 2002. The microbe-provoked infection episodes can be divided into polyclonal, monoclonal and cluster ones.

[Crimean hemorrhagic fever in the Rostov region: epidemiological zoning and the activity of natural foci].

The epidemiological zoning of the territory of the Rostov region has been made with the use of the epidemic process patterns and the data indicating the links between the landscape and the natural focus of infection. The spread of infected ticks has been established. The participation of several carrier species in the circulation of Crimean-Congo haemorrhagic fever virus has been confirmed.