Intracerebroventricular administration of creatine protects against damage by global cerebral ischemia in rat.

Although a large body of evidence shows that pretreatment of brain tissue with creatine protects against anoxic injury in vitro, only a couple of papers have investigated creatine protection in vivo, and they yielded conflicting results. We attempted to clarify how creatine may be protective in an in vivo model of global cerebral ischemia (GCI). We administered creatine either before of after GCI.

The effects of corticotropin-releasing factor on anoxia-induced changes in evoked potentials in living slices of rat olfactory cortex.

Experiments were performed to address the dynamics of evoked focal potentials in slices of rat olfactory cortex during 10-min anoxia and subsequent reoxygenation. These experiments showed that perfusion with corticotropin-releasing factor (CRF)--corticoliberin--at concentrations of 1, 10, and 100 nM had no effect on changes in EPSP parameters before or during anoxia. However, CRF (10 and 100 nM) significantly aided recovery of the amplitude and slope of EPSP during reoxygenation.

[Effect of corticotropin-releasing factor on anoxia-induced changes in evoked potentials in cultured tissue of the rat olfactory cortex].

The experiments were performed on incubated slices of the rat rostal olfactory cortex. The slices were treated with various concentrations (10(-7) to 10(-9)) of corticotropin-releasing factor before (40 min), during, and after a 10-minute anoxic episode. The parallel control experiments were carried out without CRF.

Electrophysiological effects of sustained delivery of CRF and its receptor agonists in hippocampal slices.

The corticotropin-releasing factor (CRF) is a hypothalamic peptide that regulates the release of adrenocorticotropic hormone (ATCH) and of beta-endorphin. It has been suggested that it modulates learning and memory processes in rat. However, the electrophysiological effects that CRF produces on hippocampal neurons have been so far little investigated.

[Effect of various concentrations of corticoliberin on long-term potentiation in slides of the rat olfactory cortex].

Perfusion of the rat olfactory cortex slices with 1 mcM corticotropin-releasing factor (CRF) increased the rate of initiation of the posttetanic potentiation. The latter had a shorter supporting phase than in the control. With the perfusion, with 0.