The Influence of Ionic Environment on Nucleosome-Mica Interactions Revealed via Molecular Dynamics Simulations.

Nucleosomes are the fundamental units of DNA compaction, playing a key role in modulating gene expression. As such, they are widely studied through both experimental and computational methods. While atomic force microscopy (AFM) is a powerful tool for visualizing and characterizing both canonical and modified nucleosomes, it relies on nucleosome interactions with mica surfaces.

Beyond inclusion politics: A critical discourse analysis of sex and gender in medical education.

Introduction: Medical training refers frequently to sex and gender, yet these terms are often used vaguely and interchangeably with little critical engagement. We aimed to better understand the key discourses around sex and gender in medical training.

Methods: All verbal and written instances of gendered/sexed language, occurring in lectures, slides and provided written materials, within 1 year of preclerkship medical education at a single institution in Canada were recorded autoethnographically by a medical student.

The Influence of Ionic Environment on Nucleosome-Mica Interactions Revealed via Molecular Dynamics Simulations.

Mica serves as a crucial substrate in Atomic Force Microscopy (AFM) studies for visualizing and characterizing nucleosomes. Nucleosomes interact with the negatively charged mica surface via adsorbed cations. However, the specific influences of monovalent and divalent cations on nucleosome adsorption to the mica surface remain unclear.

Modeling allosteric mechanisms of eukaryotic type II topoisomerases.

Type II topoisomerases (TopoIIs) are ubiquitous enzymes that are involved in crucial nuclear processes such as genome organization, chromosome segregation, and other DNA metabolic processes. These enzymes function as large, homodimeric complexes that undergo a complex cycle of binding and hydrolysis of two ATP molecules in their ATPase domains, which regulates the capture and passage of one DNA double-helix through a second, cleaved DNA molecule. This process requires the transmission of information about the state of the bound nucleotide over vast ranges in the TopoII complex.