Knowledge of Osteoporosis and Its Associated Factors among Public Health Professionals in a Municipal Office in Japan.

Lifelong efforts to maintain bone health are beneficial for preventing osteoporosis, and public health professionals play an important role in that. The current study aimed to assess the knowledge of osteoporosis among public health professionals in a Japanese municipal office and explored associated factors. A questionnaire was distributed to 124 eligible public health professionals in a municipal office in S City, Japan; in total, 89 individuals who returned it were analyzed.

An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography.

Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) holds great potential for structure determination of challenging proteins that are not amenable to producing large well diffracting crystals. Efficient de novo phasing methods are highly demanding and as such most SFX structures have been determined by molecular replacement methods. Here we employed single isomorphous replacement with anomalous scattering (SIRAS) for phasing and demonstrate successful application to SFX de novo phasing.

Synthesis and inhibitory activity of substrate-analog fructosyl peptide oxidase inhibitors.

Fructosyl peptide oxidases (FPOXs) play a crucial role in the diagnosis of diabetes. Their main function is to cleave fructosyl amino acids or fructosyl peptides into glucosone and the corresponding amino acids/dipeptides. In this study, the substrate-analog FPOX inhibitors 1a-c were successfully designed and synthesized.

Plasmon resonance-enhanced circularly polarized luminescence of self-assembled meso-tetrakis(4-sulfonatophenyl)porphyrin-surfactant complexes in interaction with Ag nanoparticles.

The chiroptical properties of an anionic meso-tetrakis(4-sulfonatophenyl)porphyrin (TPPS) complexed with cationic surfactants were enhanced by interaction with silver nanoparticles (AgNPs) in acidic solution. Improvement in chiroptical properties was revealed by circular dichroism (CD) and circularly polarized luminescence (CPL), with |gabs| and |glum| values reaching 0.05 and 0.

Crystallization and preliminary crystallographic analysis of two eukaryotic fructosyl peptide oxidases.

Fructosyl peptide oxidase (FPOX) catalyses the oxidation of α-glycated dipeptides such as N(α)-(1-deoxy-D-fructos-1-yl)-L-valyl-L-histidine (Fru-ValHis) and is used in the diagnosis of diabetes mellitus. Here, two thermostable mutants of FPOX, CFP-T7 and EFP-T5M, were crystallized by the sitting-drop vapour-diffusion method. The crystal of CFP-T7 belonged to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 110.