Publications by authors named "N J Pavlos"
Antisense oligonucleotides (ASOs) are a well-established therapeutic modality based on RNA interference, but low cellular uptake, limited ability to direct ASO trafficking, and a range of intracellular barriers to successful activity compromise both gene silencing outcomes and clinical translations. Herein, we demonstrate that polymers can increase ASO internalisation intracellular trafficking pathways that are distinct from lipid-based delivery reagents. For the first time, we spatially define internalisation and dissociation stages in the polymer-mediated cytosolic delivery of ASOs using Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS), which enables visualisation of ASO localisation at the organelle level.
View Article and Find Full Text PDFCell-cell fusion is an evolutionarily conserved process that is essential for many functions, including fertilisation and the formation of placenta, muscle and osteoclasts, multinucleated cells that are unique in their ability to resorb bone. The mechanisms of osteoclast multinucleation involve dynamic interactions between the actin cytoskeleton and the plasma membrane that are still poorly characterized. Here, we found that moesin, a cytoskeletal linker protein member of the Ezrin/Radixin/Moesin (ERM) protein family, is activated during osteoclast maturation and plays an instrumental role in both osteoclast fusion and function.
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- Traumatic heterotopic ossification (tHO) is the formation of extra bone in muscles and soft tissues following trauma, leading to significant challenges in patient recovery and higher healthcare costs.
- A study analyzed 188 trauma patients over 14 years, comparing those with tHO to matched control patients, revealing that tHO patients had a much longer average hospital stay (142 days vs. 61 days).
- Key factors contributing to longer hospital stays included a tHO diagnosis, use of mechanical ventilation, injuries to specific body areas, and other medical complications like pressure injuries and deep vein thrombosis.
Article Synopsis
- tHO, or traumatic heterotopic ossification, is an abnormal bone formation in soft tissues resulting from burn, neurological, or orthopedic injuries, which is not well documented in Western Australia trauma centers.
- The study aimed to assess the completeness and accuracy of ICD-10-AM diagnostic coding for tHO across four hospitals in WA, revealing that specific codes often missed identifying true cases.
- Findings showed that over one-third of actual tHO cases were not captured by specific codes, indicating discrepancies in clinical documentation within the hospital network and suggesting significant coding inaccuracies.
Objectives: Osteoarthritis (OA) is a joint disease with a heritable component. Genetic loci identified via genome-wide association studies (GWAS) account for an estimated 26.3% of the disease trait variance in humans.
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