[Effect of Cooking and Processing on Quantitation of Soybean Proteins].

We examined the effects of cooking and processing on the quantitation of soy protein in various soy-based foods. For the phosphate-buffered saline (PBS) extraction, the total protein content was measured by bicinchoninic acid assay, and the buffer extraction containing sodium dodecyl sulfate (SDS) and 2-mercaptoethanol (ME) was measured by the 2-D Quant Kit, and SDS-polyacrylamide gel electrophoresis analysis (SDS-PAGE) of each extraction was performed. Furthermore, measurements were performed by various ELISAs.

Synthesis of ε-Keggin-Type Cobaltomolybdate-Based 3D Framework Material and Characterization Using Atomic-Scale HAADF-STEM and XANES.

We describe the preparation of ε-Keggin-type cobaltomolybdate-based 3D frameworks with sodium cations, NaH[ε-CoMoMoOCo], and their characterization by high-resolution high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) and X-ray absorption fine structure (XAFS) spectroscopy. Atomic-scale HAADF-STEM images of ε-Keggin compounds were obtained for the first time, and positions of Mo and Co were confirmed. Furthermore, clear evidence of the presence of a CoO tetrahedron was obtained by X-ray absorption near-edge structure (XANES) analysis.

Arms race between weevil rostrum length and camellia pericarp thickness: Geographical cline and theory.

The geographical cline of the coevolving traits of weevil rostrum (mouthpart) length and camellia pericarp (fruit coat) thickness provides an opportunity to test the arms race theory of defense (pericarp thickness) and countermeasure (rostrum length) between antagonistically interacting species. By extending the previous model for the coevolution of quantitative traits to introduce nonlinear costs for exaggerated traits, the generation overlap, and density-dependent regulation in the host, we studied the evolutionarily stable (ES) pericarp thickness in the Japanese camellia (Camellia japonica) and the ES rostrum length in the camellia-weevil (Curculio camelliae). The joint monomorphic ES system has a robust outcome with nonlinear costs, and we analyzed how the traits of both species at evolutionary equilibrium depend on demographic parameters.

Evaluation of certain food additives and contaminants.

This report represents the conclusions of a Joint FAO/WHO Expert Committee convened to evaluate the safety of various food additives, including flavouring agents, with a view to recommending acceptable daily intakes (ADIs) and to preparing specifications for identity and purity. The Committee also evaluated the risk posed by two food contaminants, with the aim of advising on risk management options for the purpose of public health protection. The first part of the report contains a general discussion of the principles governing the toxicological evaluation and assessment of intake of food additives (in particular flavouring agents) and contaminants.

Analysis of UNEP priority POPs using HRGC-HRMS and their contamination profiles in livers and eggs of great cormorants (Phalacrocorax carbo) from Japan.

The present investigation demonstrates establishment of United Nations Environment Programme (UNEP) priority Persistent Organic Pollutants (POPs) using high-resolution gas chromatography-high-resolution mass spectrometry. Particularly, POPs analytical methods were established using native and (13)C-labeled internal standards of HCHs, HCB, cyclodienes, chlordanes, DDTs, mirex, dioxin-like PCBs, PCDDs, and PCDFs by isotope dilution technique. The relative response factor for 6-point calibration curve native standards (18 replicate analysis) were in the ranges of 0.