SNP Genotyping Characterizes the Genome Composition of the New Baisary Fat-Tailed Sheep Breed.

Lamb meat has become increasingly popular in several nations during the last few decades, especially in Kazakhstan. Due to the rising demand for lamb meat, our sheep breeders developed a new fat-tailed sheep and named the breed Baisary. Animals of the Baisary breed are characterized by a large physique, strong constitution, stretched body, deep and wide chest, medium or large-sized fat tail, long legs (height at the withers of adult rams 85-100 cm, sheep 75-90 cm), long lanceolate ears and strong hooves.

[The use of the 5'PstEJ6.6 probe containing a Ha-ras oncogene promoter in population genetics research by DNA fingerprinting].

The Mendelian inheritance of population genetic markers according to their zygosity has been established in a population investigation carried out in Kazakhstan and the northwestern region of Russia, using the 5'PstEJ6.6 DNA probe genetic variability both for persons of different nationality in a population and between two distant populations has been observed. Said procedure has proved more acceptable than the M13 phage DNA test for mass screening examinations because it involves genome analysis on the basis of DNA markers common to each population.

[A molecular genetic analysis of the 12th codon of the Ha-ras-1 proto-oncogene in human carcinoma and stomach ulcer cells].

A method of the restriction analysis by Msp I enzyme has been used to analyze the 12th codon of Ha-ras-1 protooncogene in 10 human carcinomas and in the stomach mucosa adjacent to them their 5 metastases into the regional lymph nodes and in 2 ulcers. No point mutation was found.

[Proto-oncogene expression in human carcinomas of the stomach and in the gastric mucosa of rats exposed to N-methyl-N'-nitro-N-nitrosoguanidine-induced gastric carcinogenesis].

The expression of c-Ha-ras-1, Ki-ras, N-ras, abl, src, fos and myc protooncogenes was analyzed in 13 cases of human gastric carcinomas. The transcriptional activity of both fos and myc protooncogenes was found to be disturbed in 47% and 42% of cases, respectively. An overexpression of fos protooncogenes as well as an appearance in some cases of atypical foc-mRNA transcripts were established.

[Polymorphism of restriction fragments of the Ha-ras-1 protooncogene in patients with carcinoma and ulcers of the stomach].

Ha-ras restriction fragments' length polymorphism (RFLP) in white blood cells and stomach tissues from patients with carcinoma and ulcer of the stomach was examined. Genomic DNAs were digested with Xho I, Pvu II, Pst I, Msp I, Bcn I, Mva I, Bsp RI. No Ha-ras-1 polymorphic variants specifically associated with the cancer disease were detected.