Vegetative cell fusion and a new stage in the life cycle of the Aphelida (Opisthosporidia).

The aphelids, intracellular parasitoids of algae, represent a large cluster of species sister to Fungi in molecular phylogenetic trees. Sharing a common ancestor with Fungi, they are very important in terms of evolution of these groups of Holomycota. Aphelid life cycle being superficially similar to that of Chytridiomycetes is understudied.

Extreme Genome and Nervous System Streamlining in the Invertebrate Parasite Intoshia variabili.

Orthonectida is a small, rare, and in many aspects enigmatic group of organisms with a unique life cycle and a highly simplified adult free-living stage parasitizing various marine invertebrates [1, 2]. Phylogenetic relationships of Orthonectida have remained controversial for a long time. According to recent data, they are close to Annelida, specifically to Clitellata [3-5].

[Expirience introduction of quantitative analysis of chimerism after allogenic stem cell transplantation by real-time PCR with InDel polymorphism.].

Using data obtained from domestic and foreign sources, we formed a set of primers and fluorogenic probes for analyzing twentysix specific sequence polymorphisms and one reference gene. In the course of evaluating the effectiveness of real-time PCR, using the example of one of the markers (S01a), we obtained the optimal amount of DNA per reaction (70 ng), providing a resolution of at least 0.1% of the method with the ability to estimate linear chimerism.

[Clinical and neuroimaging heterogeneity of dementia with Lewy bodies].

Aim: To study clinical characteristics of dementia with Lewy bodies (DLB) depending on neuropsychological, neuroimaging and concomitant cerebrovascular changes as well as on the character of disease onset.

Material And Methods: Forty-five patients with DLB (25 men and 20 women, men age 72.9±7.

A one-pot approach to Δ2-isoxazolines from ketones and arylacetylenes.

The sequential reaction of ketones with arylacetylenes and hydroxylamine in the presence of KOBu(t)/DMSO followed by the treatment of the reaction mixture with H(2)O and KOH leads to Δ(2)-isoxazolines in up to 88% yield.