[Use of regulators of the nitric oxide synthesis in experimental hemorrhagic shock and its infusion therapy].

In a model of volume-controlled hemorrhagic shock in rats bolus injection prior to hemorrhagic of non-selective inhibitor of the nitric oxide synthases--N-Nitro-L-Arginine at the dose 250 mg/kg promotes considerable blood flow redistribution and rapid death of animals. However the donor of nitric oxide--L-Arginine (300 mg/kg) enchances stability of animals in hemorrhagic shock. Infusion of L-Arginine (300 mg/kg) with physiological salt solution after bleeding restored cardiac function and microcirculation in the serous membrane of the small intestine of rats.

Effect of dinitrosyl iron complexes with glutathione on hemorrhagic shock followed by saline treatment.

It has been found that dinitrosyl iron complexes with glutathione (DNIC-GS) injected into the blood flow of rats at a dose of 0.05 μmoles/kg prior to hemorrhage significantly improve cardiac function under conditions of hemorrhagic shock manifested in increased stroke volume, left ventricular work and cardiac output to a level exceeding control values 1.5-fold.

Effect of selective inhibitors of nitric oxide synthesis on the course of experimental hemorrhagic shock.

Selective inhibitors of NO synthesis (derivatives of lysine, ornithine, and isothiourea) increased the efficiency of infusion therapy for experimental hemorrhagic shock in rats. These changes were related to improvement of cardiac function (increase in stroke volume, cardiac output, and left ventricular efficiency). Among the three inhibitors, N5-(1-iminoethyl)-L-ornithine dihydrochloride was most potent on this experimental model.

[Effect of dinitrosyl iron complex with glutathione as a nitric oxide donor on blood circulation in healthy animals].

It has been shown that the hypotensive action of the nitric oxide donor, the dinitrosyl complex of iron with glutathione, on the organism of healthy rats, which is caused by a decrease in the general peripherical immunity, does not impair the microcirculation and is accompanied by an enhancement of the contractile activity of the myocardium. In hypotension caused by the dinitrosyl iron complex, neither the tension of oxygen and nitrogen in the blood nor its basic-acidic status changes. Thus, the possible inhibitory action of this complex on some enzymes and proteins in the animal organism does not affect the functioning of the heart, vessels, and blood.

Study of polymerized hemoglobin in experiment.

Lyophilized polymerized hemoglobin has no group specificity and does not damage the kidney after 2 days of storage; its T/2 is 14-16 hours. P50 of solution prepared on the hemoglobin basis is 24-28 torr at 37 degrees C and pCO2, 40 torr in 0.05 M Tris-buffer, pH 7.