[Equilibrium and kinetic parameters of the interaction of peroxidase and its conjugates with specific antibodies in solution].

Horseradish peroxidase (HRP) and its conjugates with IgG (HRP-IgG) and cortisol (HRP-COR) interactions with polyvalent antibodies against peroxidase (ANTI-HRP) have been studied by homogeneous enzyme immunoassay. The total catalytic activity in o-phenylenediamine oxidation for free peroxidase or its conjugates and their immunocomplexes with antibodies against the enzyme (ANTI-HRP) has been measured. Linearization of time dependencies of the initial rate for peroxidation in the ln (v-v infinity)-time coordinates, where v and v infinity are the reaction rates at a definite moment of time and after the onset of the equilibrium, as well as in the [ANTI-HRP]/(v-v infinity)-[ANTI-HRP]0 and I/[ANTI-HRP]0k1 + k-1)-[ANTI-HRP]0 made it possible to determine the dissociation constant, Kd, and rate constant for the formation and dissociation, for the immunocomplexes k1 and k-1.

[Several indices of hemodynamics, gas exchange and exercise tolerance during treatment of ischemic heart disease with obsidan].

The analysis of the parameters of haemodynamics and gas exchange in connection with the tolerance of physical exercises in 57 patients with chronic ischaemic heart disease treated with Obsidan permitted to distinguish two variants of response to the exercises. The first is characterized by a greater increase of the cardiac output and by a slighter participation of the oxygen utilization coefficient and oxygen pulse in ensuring the oxygen regimen of work. The prescription of Obsidan is rational in the first variant of response to the physical exercises.