Inhibition of vesicle-mediated protein transport by nordihydroguaiaretic acid.

Nordihydroguaiaretic acid (NDGA) blocks intra-Golgi protein transport in a cell-free system and prolactin secretion from GH3 cells [Tagaya, M., Henomatsu, N., Yoshimori, T.

Inhibitors of vacuolar-type H(+)-ATPase suppresses proliferation of cultured cells.

We investigated effects of bafilomycin A1, a specific inhibitor of vacuolar-type H(+)-ATPase (V-ATPase), and its analogues on proliferation of various cultured cells. The proliferation of the various cell lines was suppressed by adding bafilomycin A1 to the culture medium. This inhibitory effect appeared at a concentration of nanomolar order and was dose dependent.

Inhibition of intracellular transport of newly synthesized prolactin by bafilomycin A1 in a pituitary tumor cell line, GH3 cells.

We have investigated the effects of bafilomycin A1, a specific inhibitor of vacuolar type H(+)-ATPase, on the secretion and intracellular distribution of prolactin in cultured rat pituitary tumor cells (GH3 cells). Pulse-labeling the cells with L-[35S]methionine for 5 min and subsequently incubating in chase medium containing 1 microM bafilomycin A1 showed inhibition of basal secretion of labeled prolactin. The inhibition of the secretion by the drug was clearly observed when it was added within 7.

Correlation between phospholipase A2 activity and intra-Golgi protein transport reconstituted in a cell-free system.

A wide variety of phospholipase A2 inhibitors blocks intra-Golgi protein transport reconstituted in a cell-free system. Phospholipase A2 activity detectable under the protein transport assay conditions is actually inhibited by the inhibitors. There is a good correlation between the inhibition of protein transport and that of phospholipase A2 activity.