A Target Class Ligandability Evaluation of WD40 Repeat-Containing Proteins.

Target class-focused drug discovery has a strong track record in pharmaceutical research, yet public domain data indicate that many members of protein families remain unliganded. Here we present a systematic approach to scale up the discovery and characterization of small molecule ligands for the WD40 repeat (WDR) protein family. We developed a comprehensive suite of protocols for protein production, crystallography, and biophysical, biochemical, and cellular assays.

Descriptive epidemiology and phylogenetic analysis of highly pathogenic avian influenza H5N1 clade 2.3.4.4b in British Columbia (B.C.) and the Yukon, Canada, September 2022 to June 2023.

Surveillance data from wildlife and poultry was used to describe the spread of highly pathogenic avian influenza (HPAI) H5N1 clade 2.3.4.

Pressure-Temperature-Magnetic Field Phase Diagram of Multiferroic (NH)FeCl·HO.

We combined synchrotron-based infrared absorbance and Raman scattering spectroscopies with diamond anvil cell techniques and a symmetry analysis to explore the properties of multiferroic (NH)FeCl·HO under extreme pressure-temperature conditions. Compression-induced splitting of the Fe-Cl stretching, Cl-Fe-Cl and Cl-Fe-O bending, and NH librational modes defines two structural phase transitions, and a group-subgroup analysis reveals space group sequences that vary depending upon proximity to the unexpectedly wide order-disorder transition. We bring these findings together with prior high-field work to develop the pressure-temperature-magnetic field phase diagram uncovering competing polar, chiral, and magnetic phases in this system.

Antibiotic treatment of in domestic sheep : Working at the livestock-wildlife interface in Yukon, Canada.

Domestic sheep can carry the bacterium in their upper respiratory tract, often with little effect on health and productivity. However, for bighorn sheep populations, there is a link between infection and pneumonia, poor lamb recruitment, and high fatality rate. Because of these outcomes, preventing transmission of to free-ranging wild sheep has garnered interest from both the livestock and wildlife sectors.

DNA assays for genetic discrimination of three subspecies in the United States.

Premise: To genetically discriminate subspecies of the common reed (), we developed real-time quantitative (qPCR) assays for identifying subsp. , subsp. , and subsp.