Non-viral, high throughput genetic engineering of primary immune cells using nanostraw-mediated transfection.

Transfection of proteins, mRNA, and chimeric antigen receptor (CAR) transgenes into immune cells remains a critical bottleneck in cell manufacturing. Current methods, such as viruses and bulk electroporation, are hampered by low transfection efficiency, unintended transgene integration, and significant cell perturbation. The Nanostraw Electro-actuated Transfection (NExT) technology offers a solution by using high aspect-ratio nanostraws and localized electric fields to precisely deliver biomolecules into cells with minimal disruption.

Associations between dietary fatty acids and kidney stones.

Kidney stones represent a significant global health challenge, with dietary habits playing a crucial role in their formation. This study investigates the association between dietary fatty acid intake-specifically saturated (SFA), monounsaturated (MUFA), and polyunsaturated fatty acids (PUFA)-and the prevalence of kidney stones in a U.S.

Efficient recognition of Parkinson's disease mice on stepping characters with CNN.

Parkinson's disease (PD), as the second most prevalent neurodegenerative disorder worldwide, impacts the quality of life for over 12 million patients. This study aims to enhance the accuracy of early diagnosis of PD through non-invasive methods, with the goal of enabling earlier intervention in the disease process. To this end, we constructed an open-field environment using flexible sensors under dark conditions, conducting experiments on a mouse model of Parkinson's disease alongside a normal control group.

Preoperative carcinoembryonic antigen to lymphocyte ratio serves as a prognostic predictor in elderly patients with colorectal cancer: a multicentre retrospective study.

Objectives: The aim of this study is to derive and validate a reliable indicator for predicting an increased risk of postoperative mortality in elderly patients undergoing curative resection for colorectal cancer (CRC).

Design: This study is of multicentre retrospective design.

Setting And Participants: A total of 1227 CRC patients undergoing curative resection (age ≥65 years) from three distinct cohorts were retrospective enrolled.

The Dual Function of Autophagy in Doxorubicin-induced Cardiotoxicity: Mechanism and Natural products.

Doxorubicin (DOX) is an anthracycline antitumor drug discovered in 1969, which can care for leukemia, breast cancer, lymphoma, and sarcoma. However, cardiotoxicity induced by DOX seriously limits its clinical value. The etiopathogenesis and therapeutic strategies are not unified.