In Vivo Imaging of Cobalt-Induced Ocular Toxicity in a Mouse Model.

Cobalt is a trace element, crucial for red blood cell formation and neurological function. Cobalt toxicity is often only diagnosed after severe manifestations, including visual impairment. We aimed to investigate whether optical coherence tomography (OCT) and magnetic resonance imaging (MRI) can effectively detect cobalt-induced ocular toxicity in a murine model.

Direct multi-element analysis of biological samples in dry matrix spots by PIXE.

The dried matrix spot (DMS) method, initially developed for neonatal blood screening, has gained prevalence in various research fields for its efficiency in handling small sample volumes and its adaptability to diverse analytical techniques. This study presents the results of the first systematic investigation of direct multi-element analysis in DMS of human blood and plasma samples with Particle Induced X-ray Emission (PIXE). Internal standard addition was used to address the issue of DMS heterogeneity and to eliminate the need for determining the sample volume equivalent, allowing a single-spot (single-punch) measurement.

Cobalt Toxicity Induces Retinopathy and Optic Neuropathy in Mice.

Purpose: To explore the effect of cobalt toxicity on vision.

Methods: A total of 103 wild-type (WT) mice were injected with cobalt chloride by two routes in different concentrations: single intravenous (IV) high or low doses (total, n = 43); or daily repeated intraperitoneal (IP) high (three days) or low (28 days, 56 days) dose, and low-dose cobalt with added minocycline (56 days) (total, n = 60); 10 WT mice served as a control group. An additional group of 17 immunodeficient NOD scid gamma (NSG) mice were injected IV or IP with cobalt, and 10 NSG mice served as control.

Structure-Function Correlation in Cobalt-Induced Brain Toxicity.

Cobalt toxicity is difficult to detect and therefore often underdiagnosed. The aim of this study was to explore the pathophysiology of cobalt-induced oxidative stress in the brain and its impact on structure and function. Thirty-five wild-type C57B16 mice received intraperitoneal cobalt chloride injections: a single high dose with evaluations at 24, 48, and 72 h ( = 5, each) or daily low doses for 28 ( = 5) or 56 days ( = 15).