Analytical and clinical performance of an automated immunoassay system (Immulite) for estradiol in serum.

The analytical and clinical performance of a commercial automated immunoassay system (Immulite) for estradiol (E2) in serum was evaluated. The functional sensitivity for E2 was 0.07 nmol/l, and analytical imprecision (<13%, <9% and <7% at 0.

In vitro effect of dipyrone on several peroxidase labelled immunoassays.

We studied the in vitro effect of dipyrone on the determination of free triiodothyronine (free T3), cortisol, progesterone, estradiol, carcinoembryonic antigen, human chorionic gonadotropin and alpha-fetoprotein measured with an immunoenzyme assay based on enhanced luminescence that uses peroxidase as label. We found significant interference from dipyrone (p < 0.01) in the determination of all the analytes mentioned: for progesterone and estradiol the interference was present at high doses of dipyrone; for free T3 and cortisol the minimum dipyrone concentration producing interference was 712 mumol/l and for carcinoembryonic antigen, human chorionic gonadotropin and alpha-fetoprotein 44 mumol/l.

Evaluation of Ciba Corning ACS:180 automated immunoassay system.

We evaluated the technical performance of the Ciba Corning ACS:180 automated immunoassay system for the following analytes: thyroid-stimulating hormone, free thyroxine, luteinizing hormone, follicle-stimulating hormone, prolactin, human chorionic gonadotropin, carcino-embryonic antigen, and prostate-specific antigen. The characteristics evaluated were: precision, carryover, linearity, lower limit of detection, analytical interferences, and comparison with other methods. Satisfactory results were obtained in the within-run and between-run precision studies.