[Evolution and Comparative Genomics of the pSM22 Plasmid of the IncF/MOBF12 Group].

A new plasmid, pSM22, was isolated from Serratia marcescens and sequenced. Its 43 190-bp sequence with an average GC-content of 58% contains 31 open reading frames (ORFs) which form replication, conjugation, stability, and adaptive modules. The replication module includes a site of initiation of leading-strand synthesis in plasmid replication, a replication termination site (terC), the rep A (=repA1) and repA4 genes, and the copA sequence, which codes for an antisense RNA (asRNA).

Isolation and sequence analysis of pCS36-4CPA, a small plasmid from sp. 36-4CPA.

A small plasmid designated pCS36-4CPA with a size of 5217 base pairs and G-C content of 50.74% was isolated from sp. 36-4CPA.

[Raoultella planticola, a new strain degrading 2,4,5-trichlorophenoxyacetic acid].

A new strain that degrades the herbicide 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) was isolated from soil, which was exposed to factors related to the petrochemical industry. According to its physiological, biochemical, cultural, and morphological traits, together with the sequence of the 16S rRNA gene, the strain was identified as Raoultella planticola 33-4ch. The strain could consume 2,4,5-T as a sole source of carbon and energy.

[Identification and characterization of a plasmid in strain Aeronomas hydrophila IBRB-36 4CPA carrying genes for catabolism of chlorophenoxyacetic acids].

Results of studying plasmid pAH36 in strain Aeronomas hydrophila IBRB-36 4CPA are presented. Plasmid pAH36 possesses BamHI, PstI, and HindIII restriction sites and is 5.4 kb in size.