Role of free nitrogen and oxygen radicals in the pathogenesis of lipopolysaccharide-induced endotoxemia.

We studied molecular mechanisms of changes in oxidative metabolism under conditions of experimental lipopolysaccharide-induced endotoxemia. Generation of reactive nitrogen and oxygen species in mice increased 18 h after treatment with lipopolysaccharide. These changes contributed to inactivation of enzymes and enzyme complexes (ribonucleotide reductase, NADH-ubiquinone oxidoreductase, and cytochrome c oxidase), dysfunction of the mitochondrial electron transport chain, and development of oxidative stress.

[Benefits of antioxidant agents' use for dental filling consequent to the endo-bleaching].

Efficacy of antioxidant agents' use after dental endo bleaching was studied in clinical trial. The study enrolled 169 patients, ranging age 16 to 60 years in age. The patients were randomized into 2 groups: control (63) and trial (106).

[The role of oxidative metabolism in pathogenesis of hyperbilirubinemia in infants].

The aim of the study was the investigation of free radical oxidation intensity in blood of newborn infants with hyperbilirubinemia. In infants blood the bilirubin, free nitric oxide, lipoperoxide radicals Fe(2+)-ions, methemoglobin, oxy- and desoxy hemoglobin content, erythrocytes deformability and resistance were studied. Results of the study testify the intensive generation of free nitric oxide, release of ferritin-free ferrous (Fe(2+)), intensification of lipoperoxidation, decrease of red blood cells resistance, and accumulation of methemoglobin in blood of newborn infants with hyperbilirubinemia.

[Age related changes of physical-chemical parameters of erythrocytes].

The aim of our study was to investigate age related changes of physical-chemical parameters of erythrocytes. It is known that insufficiency of erythrocytes antioxidant defence system in newborns is a keystone of peculiarities manifested in homeostasis among this group. The excess volume of reactive oxygen determines disorders related to the functioning of cation channels that are represented by decrease of K(+) ions, moderate hydration of erythrocytes and may cause accumulation of intraerythrocyte calcium.

[Plaferon LB in prevention of radiation-induced changes in lipid metabolism indices and erythrocyte deformability rate].

Oxidative damage of biological membranes plays critical role in the process of radiation-induced damages in tissues and cells. It involves reactive oxygen species generated under ionizing radiation. Development of radiation-induced oxidative stress facilitates the intensification of lipid peroxidation and fatty acid reorganization, which, in its turn, results in decreased lipid matrix fluidity, changes in biophysical properties of membranes, increased rigidity of membrane lipid-protein surface.