Molecular-genetic characteristics of hmgb1 mrna expression in blood of women with endometriosis associated with infertility.

Objective: Aim: To examine HMGB1 expression in the blood of women with endometriosis-associated infertility and to establish its role in disease progression.

Patients And Methods: Materials and Methods: We analyzed HMGB1 gene expression levels in two groups: 20 women with endometriosis-associated infertility (main group) and 10 healthy women (control group). The study was conducted at Bukovinian State Medical University and the Centre of Reproductive Medicine.

Extracellular vesicles, including large translating vesicles called midbody remnants, are released during the cell cycle.

Extracellular vesicles (EVs) play crucial roles in cell-cell communication, but the biogenesis of large EVs has remained elusive. Here, we show that the biogenesis of large EVs (>800 nm-2 µm) occurs predominantly through the completion of successful cytokinesis, and the majority of large EVs are midbody remnants (MBRs) with translation activity, and the unique marker MKLP1. Blocking the cell cycle or cytokinesis, genetically or chemically, significantly decreases MBRs and large (800 nm-2 µm), medium (500-800 nm), and small (<300 nm) EVs, suggesting that proliferative cells can also generate all sizes of EVs.

Effect of HS and cysteine homeostasis disturbance on ciprofloxacin sensitivity of Escherichia coli in cystine-free and cystine-fed minimal medium.

Endogenous HS has been proposed to be a universal defense mechanism against different antibiotics. Here, we studied the role of HS transiently generated during ciprofloxacin (CF) treatment in M9 minimal medium with sulfate or produced by E. coli when fed with cystine.

Cysteine Homeostasis Disturbance in Escherichia coli Caused by Exposure to Ciprofloxacin.

E. coli exposure to ciprofloxacin disturbs cysteine homeostasis; an increase in the intracellular concentration of cysteine is dangerous due to its ability to enhance ROS generation. Unlike wild-type bacteria, in which the cysteine content did not exceed the control level, cells of the gshA mutant lacking glutathione are characterized by increased concentration of intracellular cysteine in proportion to the concentrations of the antibiotic, despite the intensive export of cysteine into the medium.

Dynamic comparison the lower extremities length in students education in higher institutions.

Objective: Aim: Dynamic comparison of the lower limbs length, depending on the type of sport, followed by the construction of a mathematical model for predicting sports abilities.

Patients And Methods: Materials and Methods: The comparison of the lower limbs length in dynamics was carried out on 132 students of higher education institutions of Bukovyna. While the primary study was carried out during September-October 2021, next study of these same students was conducted in September-October 2022.