Motivation: tRNAs were originally considered uni-functional RNA molecules involved in the delivery of amino acids to growing peptide chains on the ribosome. More recently, the liberation of tRNA fragments from tRNAs via specific enzyme cleavage has been characterized. Detection of tRNA fragments in sequencing data is difficult due to tRNA sequence redundancy and the short length of both tRNAs and their fragments.
View Article and Find Full Text PDFObjective: To test if carbachol (CCh)-evoked Ca oscillations in freshly isolated murine detrusor myocytes are affected by β3-adrenoceptor (β-AR) modulators.
Materials And Methods: Isometric tension recordings were made from strips of murine detrusor, and intracellular Ca measurements were made from isolated detrusor myocytes using confocal microscopy. Transcriptional expression of β-AR sub-types in detrusor strips and isolated detrusor myocytes was assessed using reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time quantitative PCR (qPCR).
Am J Physiol Cell Physiol
November 2017
Rabbit corpus cavernosum smooth muscle (RCCSM) cells express ion channels that produce Ca-activated Cl () current, but low sensitivity to conventional antagonists has made its role in tone generation difficult to evaluate. We have reexamined this question using two new generation blockers, T16A-A01 and CaCC-A01. Isolated RCCSM cells were studied using the perforated patch method.
View Article and Find Full Text PDFInterstitial cells of Cajal (ICC) isolated from the rabbit urethra exhibit Ca-activated Cl currents (I ) that are important for the development of urethral tone. Here, we examined if TMEM16A (ANO1) contributed to this activity by examining the effect of "new-generation" TMEM16A inhibitors, CACC-A01 and T16A-A01, on I recorded from freshly isolated rabbit urethral ICC (RUICC) and on contractions of intact strips of rabbit urethra smooth muscle. Real-time quantitative PCR experiments demonstrated that TMEM16A was highly expressed in rabbit urethra smooth muscle, in comparison to TMEM16B and TMEM16F.
View Article and Find Full Text PDFPurpose: Muscarinic receptor mediated contractions of the detrusor rely on Ca influx through voltage-gated Ca channels but to our knowledge the mechanism linking stimulation of M3Rs to the activation of voltage dependent Ca channels has not been established. TRPC4 channels are receptor operated cation channels that couple muscarinic receptor activation to depolarization of intestinal smooth muscle cells, voltage-activated Ca influx and contraction. We investigated whether TRPC4 channels are involved in cholinergic mediated contractions of the detrusor.
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